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Merck
CN

I2891

Sigma-Aldrich

Pyrophosphatase, Inorganic from Bacillus stearothermophilus

lyophilized powder, 15-25 units/mg protein (biuret)

Synonym(s):

Inorganic Pyrophosphatase, Pyrophosphate phosphohydrolase

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About This Item

CAS Number:
EC Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
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form

lyophilized powder

specific activity

15-25 units/mg protein (biuret)

storage temp.

2-8°C

Application

Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme catalyzing the reaction PPi + H2O → 2Pi.
It plays an important role in protein, RNA, and DNA synthesis.

Other Notes

One unit will liberate 1.0 μmole of inorganic orthophosphate per min at pH 9.0 at 50 °C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Manhong Yao et al.
Molecular biology reports, 39(8), 7989-7996 (2012-04-28)
According to sequences of H(+)-pyrophosphatase genes from GenBank, a new H(+)-pyrophosphatase gene (KfVP1) from the halophyte Kalidium foliatum, a very salt-tolerant shrub that is highly succulent, was obtained by using reverse transcription PCR and rapid amplification of cDNA ends methods.
Jing-Jing Xiang et al.
Plant physiology, 159(4), 1488-1500 (2012-06-21)
Leaf rolling is an important agronomic trait in rice (Oryza sativa) breeding and moderate leaf rolling maintains the erectness of leaves and minimizes shadowing between leaves, leading to improved photosynthetic efficiency and grain yields. Although a few rolled-leaf mutants have
J Jämsen et al.
Biochemistry. Biokhimiia, 77(2), 165-170 (2012-02-22)
We earlier described CBS-pyrophosphatase of Moorella thermoacetica (mtCBS-PPase) as a novel phosphohydrolase that acquired a pair of nucleotide-binding CBS domains during evolution, thus endowing the protein with the capacity to be allosterically regulated by adenine nucleotides (Jämsen, J., Tuominen, H.
Eric E Hernández-Domíguez et al.
Plant science : an international journal of experimental plant biology, 187, 39-48 (2012-03-13)
Phosphorus is an essential element for all living cells, but its availability is often limiting in the soil. Plants have adapted to such limitation and respond to phosphorus deficiency. The soluble inorganic pyrophosphatases (PPase; EC 3.6.1.1) recycle the pyrophosphate produced
Habib Khoudi et al.
Journal of plant physiology, 169(1), 98-103 (2011-11-08)
Cadmium (Cd) is considered an extremely significant pollutant due to its high toxicity to many organisms. Plants have evolved several mechanisms to cope with Cd, the most important of which is vacuolar sequestration. Cadmium can be directly transported into vacuoles

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