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I2891

Pyrophosphatase, Inorganic from Bacillus stearothermophilus

Name: Pyrophosphatase, Inorganic from <I>Bacillus stearothermophilus</I>
Brand: SIGMA

lyophilized powder, 15-25 units/mg protein (biuret)

Synonym(s):

Inorganic Pyrophosphatase, Pyrophosphate phosphohydrolase

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About This Item

CAS Number:

9024-82-2

UNSPSC Code:

12352204

EC Number:

232-784-7

MDL number:

MFCD00131379

EC Number:

3.6.1.1 (BRENDA, IUBMB)

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form

lyophilized powder

specific activity

15-25 units/mg protein (biuret)

storage temp.

2-8°C

Application

Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme catalyzing the reaction PP_i + H₂O → 2P_i.
It plays an important role in protein, RNA, and DNA synthesis.

Other Notes

One unit will liberate 1.0 μmole of inorganic orthophosphate per min at pH 9.0 at 50 °C.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number

123K3786

013H3796

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Transgenic tobacco plants expressing ectopically wheat H⁺-pyrophosphatase (H⁺-PPase) gene TaVP1 show enhanced accumulation and tolerance to cadmium.

Habib Khoudi et al.

Journal of plant physiology, 169(1), 98-103 (2011-11-08)

Cadmium (Cd) is considered an extremely significant pollutant due to its high toxicity to many organisms. Plants have evolved several mechanisms to cope with Cd, the most important of which is vacuolar sequestration. Cadmium can be directly transported into vacuoles

Genetic manipulation of a "vacuolar" H(+)-PPase: from salt tolerance to yield enhancement under phosphorus-deficient soils.

Roberto A Gaxiola et al.

Plant physiology, 159(1), 3-11 (2012-03-22)

Regulation of pyrophosphate levels by H+-PPase is central for proper resumption of early plant development.

Ali Ferjani et al.

Plant signaling & behavior, 7(1), 38-42 (2012-02-04)

The synthesis of DNA, RNA, and de novo proteins is fundamental for early development of the seedling after germination, but such processes release pyrophosphate (PPi) as a byproduct of ATP hydrolysis. The over-accumulation of the inhibitory metabolite PPi in the

Overexpression of the halophyte Kalidium foliatum H⁺-pyrophosphatase gene confers salt and drought tolerance in Arabidopsis thaliana.

Manhong Yao et al.

Molecular biology reports, 39(8), 7989-7996 (2012-04-28)

According to sequences of H(+)-pyrophosphatase genes from GenBank, a new H(+)-pyrophosphatase gene (KfVP1) from the halophyte Kalidium foliatum, a very salt-tolerant shrub that is highly succulent, was obtained by using reverse transcription PCR and rapid amplification of cDNA ends methods.

Identification of direct forkhead box O1 targets involved in palmitate-induced apoptosis in clonal insulin-secreting cells using chromatin immunoprecipitation coupled to DNA selection and ligation.

H Y Lin et al.

Diabetologia, 55(10), 2703-2712 (2012-07-20)

The transcription factor, forkhead box (FOX)O1, is involved in fatty acid-induced apoptosis in pancreatic beta cells, but the precise mechanism is poorly understood. We aimed to identify which direct downstream targets of FOXO1 are involved in palmitate-induced apoptosis in the

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