I3269
Interleukin-9 from mouse
≥97% (SDS-PAGE and N-terminal analysis), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture
Synonym(s):
IL-9
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About This Item
UNSPSC Code:
51111800
MDL number:
biological source
mouse
recombinant
expressed in baculovirus infected Sf21 cells
assay
≥97% (SDS-PAGE and N-terminal analysis)
form
lyophilized powder
potency
0.01-0.1 ng/mL EC50
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
UniProt accession no.
storage temp.
−20°C
Gene Information
mouse ... Il9(16198)
Biochem/physiol Actions
A T cell derived growth factor.
Interleukin-9 was first identified as a T cell derived growth factor. High affinity receptors for IL-9 are located on a variety of hematopoietic cells including T cells and macrophages.
Interleukin-9 was first identified as a T cell derived growth factor. High affinity receptors for IL-9 are located on a variety of hematopoietic cells including T cells and macrophages. There is high sequence homology between mouse and human IL-9. Overall sequence homology between human and mouse IL-9 cDNAs is 56% and 67% identity at the amino acid and nucleotide levels, respectively. MO7e cells are responsive to both mouse and human IL-9, while only mouse IL-9 can stimulate mouse P40-responsive cell lines. Mouse IL-9 enhances erythroid burst formation by normal mouse bone marrow cells. In addition, mouse IL-9 induces day 15 fetal thymocyte proliferation in the presence of IL-2 and enhances the mast cell growth elicited by IL-3 or IL-4. Mouse IL-9 also supports the growth of certain helper T cell clones. Human IL-9 supports erythroid colony formation and synergizes with IL-4 in the production of IgE and IgG.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin
Analysis Note
The biological activity is measured in a cell proliferation assay using the mouse cell line TS1.
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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