IgG4, Lambda from human myeloma plasma

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. IgG4 is the least abundant of the IgG subclass. IgG4-related systemic diseases include autoimmune pancreatitis, mediastinal fibrosis and multiple sclerosis
Human myeloma IgG4, λ is purified from human plasma by fractionation, ion-exchange, and affinity chromatography procedures.

IgG is the main antibody type found in plasma and extracellular fluid and is expressed on B cell membrane. IgG is further subdivided into four subclass- IgG1, IgG2, IgG3 and IgG4. Human IgG4, λ myeloma protein is used to determine the specificity of ‘cytoplasmic human IgG subclass labelling′ assay in cloned Epstein-Barr virus cell line. This product is useful to probe and label CDR152 and can control T cell activation. Human IgG4 λ antibody can also be used in detection and isolation of human CD4+CD25+CD152+ regulatory T cells. This product has shown specificity for human.

Purified IgG4 λ from human.

The purified IgG4, λ may be used as an immunoglobulin calibrator, reference antigen, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot blot immunobinding, Western immunobloting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell binding assays. Purified IgG4, λ was used as isotype control in flow cytometry of chronic lymphocytic leukemia and mantle cell lymphoma cells. It was also used for lectin- surface plasmon resonance and IgG subclass labelling by flow cytometry at a concentration of 50 μg/ml.

Solution in 0.02 M Tris buffered saline, pH 8.0.

The purity and identity are determined by immunoelectrophoresis, indirect ELISA, and SDS-PAGE.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.