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Merck
CN

I5284

Sigma-Aldrich

Isoamylase from Pseudomonas sp.

ammonium sulfate suspension, ≥3,000,000 units/mg protein

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About This Item

EC Number:
3.2.1.68 (BRENDA, IUBMB)
MDL number:
MFCD00131418
UNSPSC Code:
12352204

Key Documents

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form

ammonium sulfate suspension

specific activity

≥3,000,000 units/mg protein

storage temp.

2-8°C

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Biochem/physiol Actions

Catalyzes the hydrolysis of (1-6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Physical form

Supplied as a suspension in 3.2 M ammonium sulfate

Other Notes

One unit causes an increase in A610 of 0.1 in 1 hour using rice starch as a substrate.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
BCBK8690V
BCBP0999V
BCBN9923V
BCBJ8668V
BCBM1588V

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the purity of Product I5284, Isoamylase from Pseudomonas sp.?

    The supplier claims the preparation gives a major band on SDS gels at approximately 71.5 kDa, and a major band on isoelectric focusing gels with a pI at approximately 5.0.

  4. Why is the preparation of Product I5284, Isoamylase from Pseudomonas sp., in ammonium sulfate?

    This enzyme is unstable to freeze-thaw cycles, so an ammonium sulfate suspension stored at 2-8 °C is used to preserve the enzyme activity and quality.

  5. What is the protein concentration of Product I5284, Isoamylase from Pseudomonas sp.?

    The protein concentration may vary from lot to lot and will be reported on our Certificates of Analysis.  The package will contain an amount of ammonium sulfate suspension that corresponds to the number of units given by the package size.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Francisco J Cinco-Moroyoqui et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 150(2), 153-160 (2008-04-09)
Three isoamylases of Rhyzopertha dominica (termed RdA70, RdA79, and RdA90 according to their relative mobility in gel electrophoresis) were isolated by ammonium sulfate fractionation and hydrophobic interaction chromatography. RdA70 and RdA79 showed an optimal pH of 7.0, whereas for RdA90
Akiko Kubo et al.
Plant physiology, 153(3), 956-969 (2010-05-08)
Functions of isoamylase-type starch-debranching enzyme (ISA) proteins and complexes in maize (Zea mays) endosperm were characterized. Wild-type endosperm contained three high molecular mass ISA complexes resolved by gel permeation chromatography and native-polyacrylamide gel electrophoresis. Two complexes of approximately 400 kD
Steven S-S Wang et al.
Bioprocess and biosystems engineering, 33(8), 1007-1015 (2010-04-08)
The current research examines the impact of agitation on deactivation of isoamylase and β-amylase under supercritical carbon dioxide (SC-CO2). Our experimental results showed that the activity of either enzyme decreased with increasing pressure or speed of agitation. The degree of
Victoria H Work et al.
Eukaryotic cell, 9(8), 1251-1261 (2010-06-22)
The accumulation of bioenergy carriers was assessed in two starchless mutants of Chlamydomonas reinhardtii (the sta6 [ADP-glucose pyrophosphorylase] and sta7-10 [isoamylase] mutants), a control strain (CC124), and two complemented strains of the sta7-10 mutant. The results indicate that the genetic
Lucio Gullo et al.
Pancreas, 38(4), 396-400 (2009-03-20)
To determine the type and frequency of pancreatic lesions detected by magnetic resonance cholangiopancreatography (MRCP) in subjects with asymptomatic pancreatic hyperenzymemia and to assess for a possible relationship between these lesions and the hyperenzymemia. From January 2005 to May 2008
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