I6505
Diethylaminoethyl–Sephacel®
aqueous ethanol suspension, 40-160 μm (wet), exclusion limit ~1,000,000 Da
Synonym(s):
DEAE-Sephacel®
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
47101511
PubChem Substance ID:
NACRES:
NA.56
form
aqueous ethanol suspension
Quality Level
technique(s)
activity assay: suitable
particle size
40-160 μm (wet)
pore size
~1,000,000 Da exclusion limit
pH
2—12
capacity
100-140 μeq/mL, gel
storage temp.
2-8°C
SMILES string
OC[C@@H]1O[C@@H](O[C@H]2[C@@H](O)[C@H](O)[C@@H](O)O[C@H]2CO)[C@@H](O)[C@H](O)[C@H]1O
InChI
1S/C12H22O11/c13-1-3-5(15)6(16)9(19)12(22-3)23-10-4(2-14)21-11(20)8(18)7(10)17/h3-20H,1-2H2/t3?,4?,5?,6?,7?,8?,9?,10-,11?,12+/m1/s1
InChI key
GUBGYTABKSRVRQ-WFVLMXAXSA-N
General description
I6505-500ML′s update product number is GE17-0500-01
Application
Diethylaminoethyl (DEAE) Sephacel® is used in protein chromatography, ion exchange chromatography, and anion exchange media. It has been used to study sperm motility, sperm capacitation and sperm-oocyte interactions. DEAE Sephacel has also been used to study patients with immunoglobulin A (IgA) nephropathy. Diethylaminoethyl–Sephacel® has been used:
- in the purification of media samples by ion-exchange chromatography
- in the isolation of glycosaminoglycans (GAG) from cultured animal cells
- in the purification of cytotoxic fractions from Tritrichomonas foetus isolates
Physical form
Suspension in 20% ethanol
Legal Information
Sephacel is a registered trademark of Cytiva
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Flam. Liq. 3
Storage Class Code
3 - Flammable liquids
WGK
WGK 1
Flash Point(F)
129.2 °F
Flash Point(C)
54 °C
Regulatory Information
危险化学品
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C M Rembold et al.
Biochimica et biophysica acta, 1500(3), 257-264 (2000-03-04)
Nitrovasodilators, high extracellular Mg(2+), and some other relaxing agents can cause smooth muscle relaxation without reductions in myosin regulatory light chain (MRLC) phosphorylation. Relaxations without MRLC dephosphorylation suggest that other regulatory systems, beyond MRLC phosphorylation, are present in smooth muscle.
Daniel R Studelska et al.
Glycobiology, 16(1), 65-72 (2005-09-17)
Glycosaminoglycans (GAGs) are linear polysaccharides made by all animal cells. GAGs bind to hundreds of proteins, such as growth factors, cytokines, chemokines, extracellular matrix components, protease inhibitors, proteases, and lipoprotein lipase, through carbohydrate and protein interactions. These interactions control many
The spectroscopic and photochemical properties of locked-15,15'-cis-spheroidene in solution and incorporated into the reaction center of <I>Rhodobacter sphaeroides</I> R-26.1.
Bautista, J.A., et al.
Photosynthesis Research, 55(1), 49-65 (1998)
V T D'Souza et al.
Biochemical and biophysical research communications, 129(3), 727-732 (1985-06-28)
An artificial chymotrypsin, with all the features of the real chymotrypsin, namely a binding site (from cyclodextrin) attached to a catalytic site containing an imidazolyl group, a carboxylate group and a hydroxyl group, has been synthesized. This artificial chymotrypsin has
Yuan-Hong Wang et al.
Acta biochimica et biophysica Sinica, 38(9), 633-638 (2006-09-06)
Extracellular alginate lyase secreted by marine Vibrio sp. YWA, isolated from decayed Laminaria japonica, was purified by a combination of ammonium sulfate precipitation and diethylaminoethyl-Sephacel column chromatography. The results show that the molecular mass of alginate lyase was approximately 62.5
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