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Merck
CN

J1000

Sigma-Aldrich

Janus Green B

Synonym(s):

3-Diethylamino-7-(4-dimethylaminophenylazo)-5-phenylphenazinium chloride, Diazin Green S, Union Green B

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About This Item

Empirical Formula (Hill Notation):
C30H31ClN6
CAS Number:
2869-83-2
Molecular Weight:
511.06
Colour Index Number:
11050
EC Number:
220-695-6
MDL number:
MFCD00011758
UNSPSC Code:
12171500

Key Documents

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composition

Dye content, ~65%

SMILES string

[Cl-].CCN(CC)c1ccc2nc3ccc(cc3[n+](-c4ccccc4)c2c1)\N=N\c5ccc(cc5)N(C)C

InChI

1S/C30H31N6.ClH/c1-5-35(6-2)26-17-19-28-30(21-26)36(25-10-8-7-9-11-25)29-20-23(14-18-27(29)31-28)33-32-22-12-15-24(16-13-22)34(3)4;/h7-21H,5-6H2,1-4H3;1H/q+1;/p-1

InChI key

XXACTDWGHQXLGW-UHFFFAOYSA-M

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Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
057K3711
025K3723
117H3622
106H3664
101K3679

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M K Dutt
Microscopica acta, 86(1), 59-68 (1982-05-01)
This communication presents informations on the staining of DNA with basic dyes, such as ethyl violet, Janus green, a new red dye obtained from Janus green, and Giemsa, belonging to different chemical groups. It has been found that DNA of
Jinling Yang et al.
Protoplasma, 231(3-4), 249-252 (2007-10-09)
Flow cytometric analysis of mitochondria isolated from maize leaves revealed two distinct rhodamine-123-stained fluorescence populations distinguishable by their main fluorescence channel. Further microscopic observation of mitochondria stained with Janus Green B and rhodamine-123 revealed the occurrence of differently sized mitochondrial
J E Yack
Journal of neuroscience methods, 49(1-2), 17-22 (1993-08-01)
Effective staining of peripheral nerves in live insects is achieved with the vital stain Janus Green B. A working solution of 0.02% Janus Green B in saline is briefly applied to the exposed peripheral nervous system. The stain is then
Jinmei Ding et al.
Electrophoresis, 32(3-4), 455-463 (2011-02-08)
Capillary zone electrophoresis (CZE) was used to determine Hela cells activity with Hela treated by 0-46 μM methylmercury (MeHg) as the apoptosis model. The treated and untreated cells were stained by four different dyes (Janus Green B, Rhodamine 123, Neutral Red
Byung-Cheon Lee et al.
Anatomical record. Part B, New anatomist, 286(1), 1-7 (2005-09-24)
A staining method has been developed for in situ and in vivo observation of a threadlike tissue afloat inside the lymphatic vessels of rabbits without adherence to the vessel wall. The existence of this novel structure was not noticed previously
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