J3895
JM109 Competent Cells
for routine DNA plasmid production
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About This Item
UNSPSC Code:
12352200
grade
Molecular Biology
form
suspension
cell transformation
competent cell type: chemically competent
transformation efficiency: >1 x 108 cfu/μg
shipped in
dry ice
storage temp.
−70°C
General description
JM109 are competent E. coli with a transformation efficiency of >1x108 cfu/μg when transformed with non-saturating amounts of pUC19 plasmid DNA.
Application
Suitable for bacterial transformations to recover high quality plasmid DNA or single-stranded bacteriophage DNA
Biochem/physiol Actions
JM109 competent E. coli contains mutations in recA1 and endA1 genes. These mutations aid in minimizing recombination and ensuring plasmid stability. The F′ factor is suitable for the growth of bacteriophages (such as M13) to obtain single stranded DNA.
Features and Benefits
- Ensures recovery of stable and high quality plasmid DNA
- Guaranteed high transformation efficiency
- Convenient pack size is sufficient for 10 transformation reactions
Other Notes
- JM109 chemically competent cells, 10 X 50 μL (J4020)
- pUC 19 control DNA (10 ng/μL), 10 μL (D2567)
Storage Class
10 - Combustible liquids
Regulatory Information
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Katherine McCaffrey et al.
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Gillian G Johnson et al.
Cancer research, 69(12), 5210-5217 (2009-06-06)
The ATM-p53 pathway plays an important role in the biology of chronic lymphocytic leukemia (CLL). Its functional integrity can be probed by exposing CLL cells to ionizing radiation (IR) and measuring levels of p53 protein and one of its transcriptional
C Yanisch-Perron et al.
Gene, 33(1), 103-119 (1985-01-01)
Three kinds of improvements have been introduced into the M13-based cloning systems. (1) New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors. Mutations introduced into these strains improve cloning