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Merck
CN

K1127

Sigma-Aldrich

Kemptide acetate salt

≥95% (HPLC), powder

Synonym(s):

Phosphate acceptor peptide

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About This Item

Empirical Formula (Hill Notation):
C32H61N13O9 · xC2H4O2
CAS Number:
1629902-50-6
Molecular Weight:
771.91 (free base basis)
Beilstein:
6263936
UNSPSC Code:
12352200
PubChem Substance ID:
24896201

Key Documents

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Assay

≥95% (HPLC)

form

powder

color

white

solubility

H2O: 20 mg/mL

storage temp.

−20°C

SMILES string

CC(O)=O.[H]N[C@@H](CC(C)C)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CCCNC(N)=N)C(N[C@H](C(N[C@@H](CO)C(N[C@@H](CC(C)C)C(NCC(O)=O)=O)=O)=O)C)=O)=O)=O

InChI

1S/C32H61N13O9.C2H4O2/c1-16(2)12-19(33)26(50)42-21(9-7-11-39-32(36)37)29(53)43-20(8-6-10-38-31(34)35)28(52)41-18(5)25(49)45-23(15-46)30(54)44-22(13-17(3)4)27(51)40-14-24(47)48;1-2(3)4/h16-23,46H,6-15,33H2,1-5H3,(H,40,51)(H,41,52)(H,42,50)(H,43,53)(H,44,54)(H,45,49)(H,47,48)(H4,34,35,38)(H4,36,37,39);1H3,(H,3,4)/t18-,19-,20-,21-,22-,23-;/m0./s1

InChI key

WRTDQEIEUYKAKV-PXILYFGCSA-N

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
061M5060V
SLBB3456V
061M5060
079K5053
029K5011

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Masatake Sano et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 794(1), 149-156 (2003-07-31)
Micellar electrokinetic chromatography (MEKC) with laser-induced fluorescence (LIF) detection has been developed for a protein kinase assay. This protein kinase assay could readily determine the phosphorylation activity of substrate peptide kemptide using cAMP-dependent protein kinase (PKA) as a model enzyme.
Role of multiple basic residues in determining the substrate specificity of cyclic AMP-dependent protein kinase.
B E Kemp et al.
The Journal of biological chemistry, 252(14), 4888-4894 (1977-07-25)
N-terminal protein sequencing for special applications.
Philip J Jackson
Methods in molecular biology (Clifton, N.J.), 211, 287-300 (2002-12-20)
L Belloni-Olivi et al.
The Biochemical journal, 315 ( Pt 2), 401-406 (1996-04-15)
In immature rat microvessels, endothelial cells and glioma cells, exposure to lead results in an increase in the level of protein kinase C in membranes. In this paper we have extended these studies to human erythrocytes and, in addition, studied
J L Maller et al.
Proceedings of the National Academy of Sciences of the United States of America, 75(1), 248-251 (1978-01-01)
A model synthetic peptide substrate of the cyclic AMP-dependent protein kinase (ATP:protein phosphotransferase; EC 2.7.1.37), Leu-Arg-Arg-Ala-Ser-Leu-Gly, closely resembling the local phosphorylation site sequence in porcine hepatic pyruvate kinase, was shown to be phosphorylated in vivo after microinjection into Xenopus oocytes.
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