grade
Molecular Biology
Assay
>99% (SDS-PAGE)
form
buffered aqueous solution
specific activity
77,000 U/mg
concentration
2,000 U/mL
shipped in
dry ice
storage temp.
−20°C
General description
Uracil-DNA Glycosylase catalyzes the hydrolysis of the N-glycosylic bond between the uracil and sugar, leaving an abasic site in uracil-containing single or double-stranded DNA. The enzyme shows no measurable activity on short oligonucleotides (<6 bases), or RNA substrates.
Application
Suitable for:
- Removing uracil from DNA
- Control of carry-over contamination in PCR
Features and Benefits
- Ultra-purification process for ultimate enzyme performance
- Highest quality specifications for ultimate product consistency
- Undetectable DNA and nuclease contamination
Physical form
Supplied in 10 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, and 50% glycerol at pH 7.5 @ 25° C
Other Notes
1 unit is defined as the amount of enzyme that catalyzes the release of 1.8 nmol of Uracil in 30 minutes from double-stranded, tritiated, Uracil containing-DNA at 37° C in 1X UDG Reaction Buffer.
Source of protein: A recombinant E. coli strain carrying the Uracil DNA Glycosylase gene from E. coli K-12.
Supplied with:KEM0001B (10X UDG Reaction Buffer)
Unit Size: 10,000 U
Storage Class Code
10 - Combustible liquids
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service