KEM0001
Uracil DNA Glycosylase (UDG)
Ultra-pure enzyme for nucleic acid modifications
grade
Molecular Biology
Assay
>99% (SDS-PAGE)
form
buffered aqueous solution
specific activity
77,000 U/mg
concentration
2,000 U/mL
shipped in
dry ice
storage temp.
−20°C
General description
Uracil-DNA Glycosylase catalyzes the hydrolysis of the N-glycosylic bond between the uracil and sugar, leaving an abasic site in uracil-containing single or double-stranded DNA. The enzyme shows no measurable activity on short oligonucleotides (<6 bases), or RNA substrates.
Application
Suitable for:
- Removing uracil from DNA
- Control of carry-over contamination in PCR
Features and Benefits
- Ultra-purification process for ultimate enzyme performance
- Highest quality specifications for ultimate product consistency
- Undetectable DNA and nuclease contamination
Physical form
Supplied in 10 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, and 50% glycerol at pH 7.5 @ 25° C
Other Notes
1 unit is defined as the amount of enzyme that catalyzes the release of 1.8 nmol of Uracil in 30 minutes from double-stranded, tritiated, Uracil containing-DNA at 37° C in 1X UDG Reaction Buffer.
Source of protein: A recombinant E. coli strain carrying the Uracil DNA Glycosylase gene from E. coli K-12.
Supplied with:KEM0001B (10X UDG Reaction Buffer)
Unit Size: 10,000 U
Storage Class Code
10 - Combustible liquids
Regulatory Information
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Laure Rittié et al.
Journal of cell communication and signaling, 2(1-2), 25-45 (2008-09-04)
Since molecular cloning has become routine laboratory technique, manufacturers offer countless sources of enzymes to generate and manipulate nucleic acids. Thus, selecting the appropriate enzyme for a specific task may seem difficult to the novice. This review aims at providing
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