KEM0006
T4 Polynucleotide Kinase
Ultra-pure enzyme for nucleic acid modifications
grade
Molecular Biology
Assay
>99% (SDS-PAGE)
form
buffered aqueous solution
concentration
10,000 U/mL
shipped in
dry ice
storage temp.
−20°C
General description
T4 Polynucleotide Kinase (PNK) catalyzes the transfer and exchange of the terminal gamma position phosphate of ATP to the 5′-hydroxyl terminus of double-and-single-stranded DNA, RNA and nucleoside 3′-monophosphate molecules. T4 PNK also exhibits 3′-phosphatase and 2′, 3′ cyclicphosphodiesterase activities.
Application
Suitable for:
- End-labeling DNA or RND for probes and DNA sequencing
- Addition of 5′ phosphates to oligonucleotides to allow subsequent ligation
- Removal of 3′ phosphoryl groups
Features and Benefits
- Ultra-purification process for ultimate enzyme performance
- Highest quality specifications for ultimate product consistency
- Undetectable DNA and nuclease contamination
Physical form
Supplied in 10 mM Tris-HCl, 50 mM KCl, 0.1 μM ATP, 1.0 mM DTT, 0.1 mM EDTA and 50% glycerol at pH 7.6 @ 25° C.
Other Notes
1 unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmol of [32P] (ATP donor) in 30 minutes at 37° C in 1X T4 Polynucleotide Kinase Reaction Buffer.
Supplied with:KEM0006B (10X T4 Polynucleotide Kinase Buffer)
Unit size: 10,000 U
Storage Class Code
10 - Combustible liquids
Regulatory Information
新产品
This item has
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Laure Rittié et al.
Journal of cell communication and signaling, 2(1-2), 25-45 (2008-09-04)
Since molecular cloning has become routine laboratory technique, manufacturers offer countless sources of enzymes to generate and manipulate nucleic acids. Thus, selecting the appropriate enzyme for a specific task may seem difficult to the novice. This review aims at providing
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service