KEM0025
E. coli DNA Ligase
Ultra-pure enzyme for nucleic acid modifications
grade
Molecular Biology
Assay
>99% (SDS-PAGE)
form
buffered aqueous solution
specific activity
20,000-28,880 U/mg
concentration
10,000 U/mL
shipped in
dry ice
storage temp.
−20°C
General description
E. coli DNA ligase catalyzes the phosphodiester bond formation between an adjacent 5′ phosphate and a 3′ hydroxyl of DNA ends, requiring NAD+ and Mg2+ as cofactors. Ligation of blunt ended DNA is extremely inefficient relative to cohesive DNA end ligation and nick sealing.
Application
Suitable for cDNA cloning by replacement synthesis.
Features and Benefits
- Ultra-purification process for ultimate enzyme performance
- Highest quality specifications for ultimate product consistency
- Undetectable DNA and nuclease contamination
Physical form
Supplied in 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA and 50% glycerol at pH 7.5 @ 25° C.
Other Notes
1 unit is defined as the amount of E.coli DNA Ligase required to ligate 50% of 100 ng DNA fragments with cohesive termini in 30 minutes at 25° C.
Source of protein: The gene encoding E. coli DNA Ligase expressed from a plasmid in E. coli.
Supplied with:KEM0025B (10X E.coli DNA Ligase Reaction Buffer)
Unit size: 2,500 U
Storage Class Code
10 - Combustible liquids
Regulatory Information
新产品
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Laure Rittié et al.
Journal of cell communication and signaling, 2(1-2), 25-45 (2008-09-04)
Since molecular cloning has become routine laboratory technique, manufacturers offer countless sources of enzymes to generate and manipulate nucleic acids. Thus, selecting the appropriate enzyme for a specific task may seem difficult to the novice. This review aims at providing
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service