KEM0028
φ29 DNA Polymerase (High Concentration)
Ultra-pure enzyme for nucleic acid modifications
grade
Molecular Biology
assay
>99% (SDS-PAGE)
form
buffered aqueous solution
specific activity
83,333 U/mg
concentration
100,000 U/mL
shipped in
dry ice
storage temp.
−20°C
General description
φ29 DNA Polymerase responsible for the replication of the Bacillus Subtilis phage φ29 (1). The enzyme is a highly processive DNA polymerase (up to 70,000 base insertions per binding event) with a powerful strand displacement activity (2) and a 3′ → 5′ proofreading exonuclease function.
Application
Suitable for Whole Genome Amplification.
Features and Benefits
- Ultra-purification process for ultimate enzyme performance
- Highest quality specifications for ultimate product consistency
- Undetectable DNA and nuclease contamination
Physical form
Supplied in 10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% TWEEN®-20, 0.5% NP-40, and 50% glycerol at pH 7.4 @ 25° C.
Other Notes
1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dTTP into acid insoluble material in 10 minutes at 30° C.
Source of protein: A recombinant E. coli strain carrying the φ29 DNA Polymerase gene from bacteriophage φ29.
Supplied with:KEM0054B (10X φ29 DNA Polymerase Reaction Buffer)
Unit size: 2,000 U
Legal Information
TWEEN is a registered trademark of Croda International PLC
Storage Class
10 - Combustible liquids
Regulatory Information
新产品
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Laure Rittié et al.
Journal of cell communication and signaling, 2(1-2), 25-45 (2008-09-04)
Since molecular cloning has become routine laboratory technique, manufacturers offer countless sources of enzymes to generate and manipulate nucleic acids. Thus, selecting the appropriate enzyme for a specific task may seem difficult to the novice. This review aims at providing
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service