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Merck
CN

L0144

Sigma-Aldrich

Lucifer Yellow CH dipotassium salt

Intracellular staining dye, powder

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About This Item

Empirical Formula (Hill Notation):
C13H9K2N5O9S2
CAS Number:
71206-95-6
Molecular Weight:
521.57
Beilstein:
5224942
MDL number:
MFCD00013445
UNSPSC Code:
12171500
PubChem Substance ID:
24896249
NACRES:
NA.77

Key Documents

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Product Name

Lucifer Yellow CH dipotassium salt, powder

form

powder

Quality Level

100

color

orange

fluorescence

λex 428 nm; λem 540 nm

storage temp.

room temp

SMILES string

[K+].[K+].NNC(=O)NN1C(=O)c2cc(cc3c(N)c(cc(C1=O)c23)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C13H11N5O9S2.2K/c14-10-5-1-4(28(22,23)24)2-6-9(5)7(3-8(10)29(25,26)27)12(20)18(11(6)19)17-13(21)16-15;;/h1-3H,14-15H2,(H2,16,17,21)(H,22,23,24)(H,25,26,27);;/q;2*+1/p-2

InChI key

HCWYSCVNSCIZCN-UHFFFAOYSA-L

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General description

Lucifer yellow CH is a fixable dye with an excitation and emission spectrum of 430 nm and 540 nm, respectively.LuciferYellow CH (LYCH) is a small, hydrophilic molecule, and highly fluorescent dye that is used for intracellular staining for the studies conducted on various types of cells. It acts as a marker for the detection of well-established tight junctions in smooth muscles, glia, and interstitial cells of Cajal (ICC).

Application

Lucifer yellow CH dipotassium salt has been used:
  • to visualize cell morphology
  • to assess intercellular communication activity through gap junction by scrape-loading assay
  • as a component of the intracellular solution, which filled the dendrites for the visualization of neuronal morphology
  • in the intracellular solution, to intracellularly label recorded neurons

Features and Benefits

Highly fluorescent dye, useful in marking nerve cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000438866
0000438866
SHBR8105
SHBR1397
SHBQ2310

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Menachem Hanani
Journal of cellular and molecular medicine, 16(1), 22-31 (2011-07-12)
The fluorescent dye Lucifer yellow (LY) was introduced in 1978, and has been extremely useful in studying cell structure and communications. This dye has been used mostly for labelling cells by intracellular injection from microelectrodes. This review describes the numerous
Kun-Yi Hsin et al.
Nucleic acids research, 39(Database issue), D1042-D1048 (2010-11-06)
We present the relational database EDULISS (EDinburgh University Ligand Selection System), which stores structural, physicochemical and pharmacophoric properties of small molecules. The database comprises a collection of over 4 million commercially available compounds from 28 different suppliers. A user-friendly web-based
A co-culture model for determining the target specificity of the de novo generated retinal ganglion cells
Teotia P, et al.
Bio-protocol, 7(7) (2017)
The morphology and intrinsic excitability of developing mouse retinal ganglion cells
Qu Juan and Myhr K L
PLoS ONE, 6(7), e21777-e21777 (2011)
Connexin-43 downregulation in G2/M phase enriched tumour cells causes extensive low-dose hyper-radiosensitivity (HRS) associated with mitochondrial apoptotic events
Ghosh S, et al.
Cancer letters, 363(1), 46-59 (2015)
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