L0382
Lipase from porcine pancreas
Type VI-S, ≥20,000 units/mg protein, lyophilized powder
Synonym(s):
PPL, Triacylglycerol acylhydrolase, Triacylglycerol lipase
type
Type VI-S
Quality Level
form
lyophilized powder
specific activity
≥20,000 units/mg protein
composition
Protein, 40-70%
shipped in
wet ice
storage temp.
−20°C
InChI
1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
InChI key
QWZUIMCIEOCSJF-CHHCPSLASA-N
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General description
Lipase from porcine pancreas (PPL) or porcine pancreatic lipase is a small globular protein with N-terminal α/β type fold. It comprises catalytic triad Ser, Asp, and His residues and a C-terminal β-sandwich domain. PPL belongs to a subclass of the carboxylesterases family.
Application
Lipase from porcine pancreas has been used:
- in lipase inhibition assay with plant extracts
- in in vitro digestion gelatinized starch/fiber mixtures
- with simulated intestinal fluid to mimic the digestive tract condition of by L. fermentum PC1 in fermented oats
Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.
Biochem/physiol Actions
Lipase from porcine pancreas (PPL) catalyzes the hydrolysis of fats (lipids). It plays an essential role in the digestion, transport, and processing of dietary lipids (e.g. triglycerides, fats, oils) in most living organisms. PPL is cost-effective and is useful in biotransformation reactions. It displays high selectivity, and catalytic activity. PPL is also used in the detergent formulation for removing oil stains from clothes.
Lipases catalyze the hydrolysis of esters in aqueous solutions and the synthesis of esters in non-aqueous solutions. They also produce hydroxyl and carboxylic groups through the hydrolysis of ester linkages in poly(ethylene terephthalate).
Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Analysis Note
Protein determined by biuret.
Other Notes
One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.7 at 37 °C using olive oil.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
低风险生物材料
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Allan K Hardacre et al.
Carbohydrate polymers, 123, 80-88 (2015-04-07)
An in vitro system was used to determine if the addition of insoluble or soluble fibre to aqueous suspensions of gelatinised starch affected the rate at which the starch was digested. Pre-gelatinised potato or corn starch suspensions were digested with
Properties and biotechnological applications of porcine pancreatic lipase
Mendes AA, et al.
Journal of Molecular Catalysis. B, Enzymatic, 78 (2012)
Irushika T Fernando et al.
Food science & nutrition, 7(2), 425-432 (2019-03-09)
This study investigated the effect of boiling on the inhibitory action of spices on digestive enzymes. Unboiled extracts of Trigonella foenum-graecum (seed) (25.42%), Myristica fragrans (seed) (22.70%), and Cuminum cyminum (seed) (19.17%) showed significantly (p < 0.05) a higher lipase inhibitory activity
Hua Zhao et al.
PloS one, 9(12), e114385-e114385 (2014-12-30)
Pancreatic lipase plays a key role in intestinal digestion of feed fat, and is often deficient in young animals such as weaning piglets. The objective of this study was to express and characterize a partial codon optimized porcine pancreatic lipase
M D Yago et al.
The British journal of nutrition, 78(1), 27-39 (1997-07-01)
The aim of the present study was to investigate in human subjects whether or not the ingestion of two liquid meals that differed only in their fatty acid composition (due to the addition of olive oil (group O) or sunflowerseed
Articles
Lipid Induced Insulin Resistance
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