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Merck
CN

L0651

Lectin from Lycopersicon esculentum (tomato)

biotin conjugate, lyophilized powder

Synonym(s):

Lycopersicon esculentum agglutinin, LEA

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About This Item

UNSPSC Code:
12352200
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conjugate

biotin conjugate

form

lyophilized powder

composition

Lectin, ≥20%

extent of labeling

≥3 mol biotin per mol lectin

storage temp.

−20°C

Biochem/physiol Actions

LEA is not blood group specific, but has an affinity for N-acetyl-β-D-glucosamine oligomers. The lectin is a glycoprotein containing approx. equal amounts of protein and carbohydrate and is reported to inhibit the mitogenic activity of phytohemagglutinin from Phaseolus vulgaris. This lectin binds endothelial cells and is used to visualize the microvasculature.

Physical form

Contains NaCl

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 7.3, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hour incubation at 25 °C.

Storage Class

13 - Non Combustible Solids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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