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Merck
CN

L1034

Sigma-Aldrich

Lectin from Helix pomatia

FITC conjugate, buffered aqueous solution

Synonym(s):

Helix pomatia agglutinin, HPA

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About This Item

UNSPSC Code:
12352200
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conjugate

FITC conjugate

form

buffered aqueous solution

potency

<1 μg per mL agglutination activity

composition

Protein, ≥0.5 mg/mL

extent of labeling

2-5 mol FITC per mol protein

storage temp.

−20°C

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Biochem/physiol Actions

HPA has anti-A human blood group specificity and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues. Conjugates are prepared from affinity purified lectin.

Physical form

Solution in 0.01 M Tris, pH 8.0, containing 0.15 M NaCl and 0.1% sodium azide

Analysis Note

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hour incubation at 25 °C.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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