L1287
Lectin from Dolichos biflorus (horse gram)
peroxidase conjugate, lyophilized powder
Synonym(s):
Dolichos biflorus agglutinin, DBA
conjugate
peroxidase conjugate
form
lyophilized powder
peroxidase activity
10-30 units/mg protein
composition
Protein, ~90% biuret
storage temp.
−20°C
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Biochem/physiol Actions
DBA has anti-A1 human blood group specificity, useful for distinguishing between A1 and A2 blood types. DBA has strong anti-Cad activity, and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues. Conjugates are prepared from affinity purified lectin.
Physical form
Contains sodium citrate
Preparation Note
Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan, which favors low molecular weight conjugates.
Other Notes
One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Laura Woods et al.
Nature communications, 8(1), 352-352 (2017-09-07)
Mammalian reproductive performance declines rapidly with advanced maternal age. This effect is largely attributed to the exponential increase in chromosome segregation errors in the oocyte with age. Yet many pregnancy complications and birth defects that become more frequent in older
A simple method for the preparation of enzyme-antibody conjugates.
M J O'Sullivan et al.
FEBS letters, 95(2), 311-313 (1978-11-15)
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