L1412
Lysing Enzymes from Trichoderma harzianum
lyophilized powder
Synonym(s):
Glucanex®
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About This Item
UNSPSC Code:
12352204
Specific activity:
≥10 units/g
form
lyophilized powder
specific activity
≥10 units/g
application(s)
diagnostic assay manufacturing
storage temp.
2-8°C
Application
Lysing Enzymes from Trichoderma harzianum is suitable retrieve DNA plugs from agarose gels. It has been used:
- for protoplast isolation
- for hydrolyzing poly(1-3)-glucose of the yeast cell wall glucan prior to yeast spheroplast transformation
- for the lysis of Aspergillus niger vegetative cells
Biochem/physiol Actions
Lysing Enzymes from Trichoderma harzianum is called Glucanex. It comprised of mixture of isoenzymes with β-1, 3 glucanase activity. Lysing Enzymes from Trichoderma harzianum hydrolyzes yeast cell wall oligosaccharides to glucan for protoplast preparation. Its stability is influenced by pH changes.
Analysis Note
Contains β-glucanase, cellulase, protease, and chitinase activities
Other Notes
One unit will hydrolyze casein to produce color equivalent to 1.0 micromole (181 μg) of tyrosine per minute at ph 7.5 at 37 deg C (color byfolin-ciocalteu reagent).
Legal Information
A product of Novozyme Corp.
Glucanex is a registered trademark of Novozymes Corp.
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Influence of protoplast fusion between two Trichoderma spp. on extracellular enzymes production and antagonistic activity
Hassan MM
Biotechnology, Biotechnological Equipment, 28(6), 1014-1023 (2014)
Divya Ramalingam Iyer et al.
PLoS genetics, 13(8), e1006958-e1006958 (2017-08-15)
In response to DNA damage during S phase, cells slow DNA replication. This slowing is orchestrated by the intra-S checkpoint and involves inhibition of origin firing and reduction of replication fork speed. Slowing of replication allows for tolerance of DNA
Identification of InuR, a new Zn (II) 2Cys6 transcriptional activator involved in the regulation of inulinolytic genes in Aspergillus niger
Yuan XL, et al.
Molecular Genetics and Genomics, 279(1), 11-26 (2008)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| L1412-10G | 04061835557448 |
| L1412-25G | 04061835518180 |
| L1412-5G | 04061833949412 |