L1900
Luria broth base, Miller′s modified
Tryptone, yeast extract and sodium chloride
Synonym(s):
Lennox broth base, modified, Luria-Bertani broth base, modified, Lysogeny broth base, modified
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About This Item
NACRES:
NA.72
UNSPSC Code:
41106200
Product Name
Luria broth base, Miller′s modified, Tryptone, yeast extract and sodium chloride
form
powder
technique(s)
microbiological culture: suitable
application(s)
agriculture
microbiology
Quality Level
Application
Luria broth base, Miller′s modified has been used as a nutrient culture medium for E-coli cells.
Luria broth base (LB), Miller (L1900) is a bacteria growth mixture of sodium chloride (0.5g/L); tryptone (10g/L) and yeast extract (5g/L) typically combined with agar (15g/L).
Luria broth base (LB), Miller (L1900) is a bacteria growth mixture of sodium chloride (0.5g/L); tryptone (10g/L) and yeast extract (5g/L) typically combined with agar (15g/L).
General description
Luria broth (LB) is a nutritionally rich bacterial culture medium that is commonly used for maintaining and cultivating laboratory recombinant strains of E. coli. It has potential applications in molecular microbiology methods such as the preparation of plasmid DNA and recombinant proteins. Luria broth base, Miller′s modified is used to culture members of the family Enterobacteriaceae and for coliphage plaque assays.
Other Notes
Contains: Tryptone, yeast extract and sodium chloride.
Preparation Note
Recommended use at 15.5 g per liter.
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Facile monitoring and evaluation of bacteria in a fermentation process using perfusion chromatography and polarimetry detection
Berthod A, et al.
Journal of Separation Science, 26.0, 20-28 (2003)
Facile monitoring and evaluation of bacteria in a fermentation process using perfusion chromatography and polarimetry detection
Berthod A, et al.
Journal of Separation Science, 26(1-2), 20-28 (2003)
Plasmid DNA topology assayed by two-dimensional agarose gel electrophoresis
Schvartzman JB, et al.
Journal of Separation Science, 121-132 (2013)
Plasmid DNA topology assayed by two-dimensional agarose gel electrophoresis
Schvartzman JB, et al.
Journal of Separation Science, 121-132 (2013)
Plasmid DNA topology assayed by two-dimensional agarose gel electrophoresis
Schvartzman JB, et al.
Journal of Separation Science, 121-132 (2013)
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