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Merck
CN

L2263

Lectin from Limulus polyphemus (horseshoe crab)

lyophilized powder

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About This Item

UNSPSC Code:
12352200
MDL number:
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form

lyophilized powder

potency

<16 μg per mL agglutination activity (with blood group B erythrocytes)

storage temp.

−20°C

Biochem/physiol Actions

Agglutinates Staphylococcus aureus cells, as well as human and horse erythrocytes.
Major affinity is for N-acetylated D-hexosamines, but is reported to have anti-galactan specificity.1

Packaging

Package size based on protein content

Physical form

Lyophilized powder containing Tris succinate, NaCl and calcium acetate

Preparation Note

Purified by affinity chromatography.

Analysis Note

Agglutination activity is expressed in μg per mL and is determined from serial dilutions in phosphate buffered saline, pH 7.4, containing 0.1 mM CaCl2, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group B erythrocytes. Bacterial agglutination is determined from serial dilutions of a 1 mg/ml solution in 0.01 M Tris, 0.15 M NaCl, pH 7.4, 0.01M CaCl2 with 1 mg/ml albumin and 0.92% TWEEN®.

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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