L2263
Lectin from Limulus polyphemus (horseshoe crab)
lyophilized powder
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About This Item
MDL number:
UNSPSC Code:
12352200
form
lyophilized powder
potency
<16 μg per mL agglutination activity (with blood group B erythrocytes)
storage temp.
−20°C
Biochem/physiol Actions
Agglutinates Staphylococcus aureus cells, as well as human and horse erythrocytes.
Major affinity is for N-acetylated D-hexosamines, but is reported to have anti-galactan specificity.1
Major affinity is for N-acetylated D-hexosamines, but is reported to have anti-galactan specificity.1
Packaging
Package size based on protein content
Physical form
Lyophilized powder containing Tris succinate, NaCl and calcium acetate
Preparation Note
Purified by affinity chromatography.
Analysis Note
Agglutination activity is expressed in μg per mL and is determined from serial dilutions in phosphate buffered saline, pH 7.4, containing 0.1 mM CaCl2, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group B erythrocytes. Bacterial agglutination is determined from serial dilutions of a 1 mg/ml solution in 0.01 M Tris, 0.15 M NaCl, pH 7.4, 0.01M CaCl2 with 1 mg/ml albumin and 0.92% TWEEN®.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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