L2280
Monoclonal Anti-Mouse Lambda Light Chains
clone 9A8, ascites fluid
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About This Item
MDL number:
UNSPSC Code:
12352203
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
secondary antibodies
clone
9A8, monoclonal
contains
15 mM sodium azide
technique(s)
flow cytometry: suitable
indirect ELISA: 1:5,000
indirect immunofluorescence: suitable
radioimmunoassay: suitable
western blot: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
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Immunogen
murine myeloma protein M315.
Biochem/physiol Actions
The antibody recognizes an epitope located in the Vλ2315 domain that is also expressed on λ1 and λ3 chains. There is no cross-reactivity with the mouse κ light chain. The antibody binds isolated chains and complete immunoglobulins of both normal serum and myeloma proteins. In addition, this antibody is easily purified on protein G immunosorbents and may be biotinylated or FITC-conjugated without loss of binding capacity.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Lu Wang et al.
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A V Popov et al.
The Journal of experimental medicine, 189(10), 1611-1620 (1999-05-20)
Transgenic mice carrying a 380-kb region of the human immunoglobulin (Ig) lambda light (L) chain locus in germline configuration were created. The introduced translocus on a yeast artificial chromosome (YAC) accommodates the most proximal Iglambda variable region (V) gene cluster
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