L2662
Lectin from Viscum album (European mistletoe)
lyophilized powder
Synonym(s):
Viscum album agglutinin, VAA
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About This Item
UNSPSC Code:
12352200
MDL number:
form
lyophilized powder
potency
<16 μg/mL agglutination activity
composition
Protein, ~50% Lowry
storage temp.
−20°C
Biochem/physiol Actions
VAA is not blood group specific, but has an affinity for terminal β-D-galactosyl residues. VAA inhibits protein synthesis similarly to Ricin (RCA60) and inhibits allergen induced histamine release in vitro from human leukocytes.
Physical form
Highly purified, lyophilized powder containing phosphate buffer salts. Package size based on protein content.
Preparation Note
Purified by affinity chromatography using a modification of the method of Ziska.
Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.
signalword
Danger
Hazard Classifications
Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral - Resp. Sens. 1 - Skin Sens. 1
Storage Class
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
ppe
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
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P Ziska et al.
Experientia, 34(1), 123-124 (1978-01-15)
A lectin from Viscum album which specifically binds to D-galactose was isolated by affinity chromatography on O-lactosyl-, O-galactosyl-polycarylamide or hydrolized sepharose 4 B. Some serological and physicochemical properties of the agglutination are reported.
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