L2766
Lectin from Datura stramonium (jimson weed, thorn apple)
lyophilized powder
Synonym(s):
Datura stramonium lectin, DSL
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About This Item
Form:
lyophilized powder
form
lyophilized powder
potency
≤10 μg per mL agglutination activity
composition
Protein, ≥60% biuret
impurities
salt, essentially free
storage temp.
−20°C
Quality Level
General description
Lectin is present in several plants and some animals. It is a tetramer composed of identical sub-units.
Application
Lectin has been used in differential plating to separate Sertoli cells from the isolated cell population. It has also been used in differential plating of spermatogonial stem cells.
Biochem/physiol Actions
DSL is not blood group specific but has an affinity for oligomers of N-acetyl-glucosamine and N-acetyllactosamine. The lectin is a glycoprotein containing approx. 35% carbohydrate.
Lectin identifies and binds to carbohydrate complexes that extend from glycolipids and glycoproteins. It also binds to free-floating glycans, such as monosaccharides.
Preparation Note
Purified by affinity chromatography
Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution in phosphate buffered saline, pH 6.8. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
Highly purified.
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
常规特殊物品
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Haike Ghazarian et al.
Acta histochemica, 113(3), 236-247 (2010-03-05)
This review is intended for general readers who would like a basic foundation in carbohydrate structure and function, lectin biology, and the implications of glycobiology in human health and disease, particularly in cancer therapeutics. These topics are among the hundreds
Comparing the stemness and morphobiometry of spermatogonial stem cells from Doom pig on different days of culture
Kumar K K, et al.
Journal of Orofacial Sciences, 4(1), 20-20 (2012)
A glycobiology review: carbohydrates, lectins and implications in cancer therapeutics
Ghazarian H, et al.
Acta Histochemica, 113(3), 236-247 (2011)
Isolation and enrichment of putative spermatogonial stem cells from ram (Ovis aries) testis
Binsila K B, et al.
Animal Reproduction Science, 196(3), 9-18 (2018)
Kristina M Thumfart et al.
Frontiers in genetics, 13, 1024805-1024805 (2022-11-11)
Sertoli cells are somatic cells in testis essential for spermatogenesis, that support the development, maturation, and differentiation of germ cells. Sertoli cells are metabolically highly active and physiologically regulated by external signals, particularly factors in the blood stream. In disease
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