L3001
Lipase from wheat germ
Type I, lyophilized powder, 5-15 units/mg solid
Synonym(s):
Triacylglycerol acylhydrolase, Triacylglycerol lipase
biological source
wheat (germ)
Quality Level
type
Type I
form
lyophilized powder
specific activity
5-15 units/mg solid
UniProt accession no.
storage temp.
2-8°C
InChI
1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
InChI key
QWZUIMCIEOCSJF-CHHCPSLASA-N
Gene Information
wheat ... lipase(543006)
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General description
Lipases belong to the class of hydrolases. Microbes of plant and animal basis mainly produce lipase enzyme. It can also be produced by marine organisms.
Application
Lipase from wheat germ has been used:
- to prepare enzyme solution for the biodegradability test
- to treat diacetates of Mep-Xylp, to generate monoacetylated xylosides
- to test its efficacy in selenium speciation analysis and to determine the contribution that impurities could make to Se determination in real samples
Biochem/physiol Actions
Lipases are used in detergent, food, leather, pharmaceutical and dairy industries. It has several characteristics like substrate specificity, stereospecificity and regioselectivity. It also participates in various bioconversion reactions like, hydrolysis, esterification, acidolysis and aminolysis.
Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).
Preparation Note
Prepared essentially by method of Singer, T.P., J. Biol. Chem., 174, 11 (1948).
Analysis Note
Contains acid phosphatase.
Other Notes
One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.4 at 37 °C. (This is equivalent to approx. 10 microliters of CO2 in 30 minutes.)
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
动植物源性产品
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Ondřej Dlouhý et al.
Cells, 10(9) (2021-09-29)
Build-up of the energized state of thylakoid membranes and the synthesis of ATP are warranted by organizing their bulk lipids into a bilayer. However, the major lipid species of these membranes, monogalactosyldiacylglycerol, is a non-bilayer lipid. It has also been
Marine fungal and bacterial isolates for lipase production: A comparative study
Advances in Food and Nutrition Research, 78, 71-94 (2016)
Preparation and characterization of poly (ethylene glycol)-crosslinked reacetylated chitosans
Dal P A, et al.
Carbohydrate Polymers, 42(2), 201-206 (2000)
Ondřej Dlouhý et al.
Cells, 10(9) (2021-09-29)
In Part I, by using 31P-NMR spectroscopy, we have shown that isolated granum and stroma thylakoid membranes (TMs), in addition to the bilayer, display two isotropic phases and an inverted hexagonal (HII) phase; saturation transfer experiments and selective effects of
Lipase: An Industrial Enzyme Through Metagenomics
Advances in Food and Nutrition Research, 78, 71-94 (2018)
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