L3544
Anti-Lamin A−Atto 647N antibody produced in rabbit
1.5-3 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-LAMA, Anti-LMNA, Anti-LNMI, Anti-Lamin A/C
biological source
rabbit
Quality Level
conjugate
Atto 647N conjugate
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
human
concentration
1.5-3 mg/mL
technique(s)
direct immunofluorescence: 5-10 μg/mL using human HeLa cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... LMNA(4000)
General description
Lamin A is a structural protein of the nuclear lamina. The nuclear lamina is a meshwork of intermediate filaments that underlies the inner face of the nuclear envelope. The precursor of lamin A, prelamin comprises 98 unique amino acids and also harbors the farnesyl group in C-terminus post-synthesis. The cleavage of the farnesyl group containing end 18 amino acids results in mature Lamin A. Lamin A is mapped to human chromosome 1q21.2.
Application
Anti-Lamin A−Atto 647N antibody produced in rabbit may be used in direct immunofluorescence.
Biochem/physiol Actions
Anti-Lamin A-Atto 647N recognizes human Lamin A.
Lamin A is cleaved into a 47 kDa fragment during apoptosis. Lamin A cleavage seems to be essential for chromatin condensation and nuclear disassembly in apoptosis. Mutations in Lamin A and C have been linked to a variety of rare human diseases including muscular dystrophy, lipodystrophy, cardiomyopathy, neuropathy and progeroid syndromes (collectively termed laminopathies) and to premature aging (Hutchinson-Gilford progeria syndrome).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Protect from prolonged exposure to light. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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The lamin protein family
Dittmer TA and Misteli T
Genome Biology, 12(5), 1-14 (2011)
The nuclear lamina and inherited disease
Worman HJ and Courvalin JC
Trends in Cell Biology, 12(12), 591-598 (2002)
Ning-Ang Liu et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 29(6), 2514-2525 (2015-03-04)
DNA double-strand breaks (DSBs) are the major lethal lesion induced by ionizing radiation (IR). RAD51-dependent homologous recombination (HR) is one of the most important pathways in DSB repair and genome integrity maintenance. However, the mechanism of HR regulation by RAD51
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