L4285
Listeria identification agar base
pH 7.0±0.2 (25 °C)
Synonym(s):
PALCAM, Polymyxin Acriflavin Lithium-Chloride Ceftazidime Aesculin Mannitol Agar
pH
7.0±0.2 (25 °C)
Application
Recommended for the selective isolation and identification of Listeria spp.
Other Notes
Components (g/L)
Peptic Digest of Animal Tissue 23.00
Starch 1.00
Sodium Chloride 5.00
Mannitol 10.00
Ammonium Ferric Citrate 0.50
Esculin 0.80
Dextrose 0.50
Lithium Chloride 15.00
Phenol Red 0.08
Agar 13.00
Peptic Digest of Animal Tissue 23.00
Starch 1.00
Sodium Chloride 5.00
Mannitol 10.00
Ammonium Ferric Citrate 0.50
Esculin 0.80
Dextrose 0.50
Lithium Chloride 15.00
Phenol Red 0.08
Agar 13.00
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Van Nettern, P., et al.
Letters in Applied Microbiology, 12, 20-20 (1991)
P van Netten et al.
International journal of food microbiology, 8(4), 299-316 (1989-07-01)
A selective and differential medium (PALCAM agar) was elaborated for the isolation and enumeration of Listeria monocytogenes. PALCAM is based on Columbia agar with 0.05% glucose made selective by the addition of 0.001% polymyxin B, 0.0005% acriflavin, 1.5% lithium chloride
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