L4889
Lectin from Ulex europaeus (gorse, furze)
TRITC conjugate, lyophilized powder
Synonym(s):
Ulex europaeus agglutinin, UEA-I
conjugate
TRITC conjugate
form
lyophilized powder
potency
<10 μg/mL agglutination activity
composition
Protein, ~10% Lowry
extent of labeling
4-8 mol TRITC per mol lectin
storage temp.
−20°C
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Biochem/physiol Actions
Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.
Packaging
Package size based on protein content.
Physical form
Contains potassium phosphate buffer salts and sodium chloride
Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.
Other Notes
Conjugates are prepared from affinity purified lectin.
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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