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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-942-5
MDL number:
EC Number:
Specific activity:
10-40 units/mg protein (Bradford)
Quality Segment
type
Type IV-S
form
ammonium sulfate suspension
specific activity
10-40 units/mg protein (Bradford)
storage temp.
2-8°C
General description
Leucine Aminopeptidase belongs to the family of metalloprotease.
Application
Biochem/physiol Actions
Leucine Aminopeptidase possesses broad substrate-specificity. It hydrolyzes peptides up to the proline residue and does not continue to degrade beyond proline.
Physical form
Suspension in 3.5 M (NH4)2SO4 solution, pH 7.7, containing 10 mM MgCl2
Other Notes
One unit will hydrolyze 1.0 μmole of L-leucine-p-nitroanilide to L -leucine and p-nitroaniline per min at pH 7.2 at 37 °C. (At 25 °C, approx. 40% of the activity at 37 °C is obtained.) The activity obtained using L-leucine p-nitroanilide as substrate is 2-5 times that obtained with L-leucinamide as substrate.
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Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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