L5037
Leptin from rat
≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder
Synonym(s):
OB
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About This Item
Biological source:
Escherichia coli
Form:
lyophilized powder
Assay:
≥97% (SDS-PAGE)
biological source
Escherichia coli
recombinant
expressed in E. coli
sterility
non-sterile
assay
≥97% (SDS-PAGE)
form
lyophilized powder
potency
≤0.8 ng/mL ED50
mol wt
16 kDa
impurities
≤1.0 EU/μg endotoxin
UniProt accession no.
shipped in
ambient
storage temp.
−20°C
Quality Level
Gene Information
rat ... Lep(25608)
Application
Leptin from rat has been used:
- to check the effect of intranasal nerve growth factor (NGF) on NGF activity in the spinal cord of Sprague-Dawley rats
- for validation of labelling for leptin-bearing cells of Sprague-Dawley rats
- to study the effect of leptin on STAT3 (signal transducer and activator of transcription 3) phosphorylation in neural population of Sprague-Dawley rats
- to study leptin response towards chemoreflex in nucleus of the solitary tract
- in rats, to check the effect of association between leptin and neuronal nitric oxide pathway on penicillin-induced epileptiform activity
Biochem/physiol Actions
Hormone produced primarily in adipocytes; primary site of action appears to be on neurons in the hypothalamus that are involved in regulating energy balance, appetite, and body weight.
In rodents with uncontrolled diabetes mellitus, leptin is crucial for normalizing hyperglycemia. In the digestive tract, it also participates in the absorption of dietary molecules via controlling transporters activity.
Leptin is a hormone produced primarily in adipocytes, although leptin mRNA has also been identified in placenta and fetal tissues, gastric tissue and liver. Its primary site of action appears to be on neurons in the hypothalamus that are involved in regulating energy balance, appetite, and body weight. Leptin increases the production of nitric oxide in endothelial cells and stimulates angiogenesis in vitro and in vivo.
Human and mouse leptin share ~84% sequence identity.
Human and mouse leptin share ~84% sequence identity.
Physical form
Lyophilized from a 0.2 μm filtered solution with 4.5 mM NaHCO3.
Analysis Note
The biological activity is measured by its ability to induce proliferation of leptin-dependent human OB-R transfected murine BAF3 cells.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
涉药品监管产品
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Gregory J Morton et al.
The Journal of clinical investigation, 125(12), 4587-4591 (2015-11-04)
Leptin administration restores euglycemia in rodents with severe insulin-deficient diabetes, and recent studies to explain this phenomenon have focused on the ability of leptin to normalize excessive hypothalamic-pituitary-adrenal (HPA) axis activity. Here, we employed a streptozotocin-induced rat model (STZ-DM) of
Carmen Fanjul et al.
Journal of physiology and biochemistry, 71(2), 311-317 (2015-05-04)
Leptin is secreted into the digestive tract and contributes to the absorption of dietary molecules by regulating transporters activity. Here, we studied the effect of luminal leptin on the intestinal transport of L-glutamate, an important component of human diet. We
John Ciriello et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 303(7), R727-R736 (2012-08-24)
Circulating levels of leptin are elevated in individuals suffering from chronic intermittent hypoxia (CIH). Systemic and central administration of leptin elicits increases in sympathetic nervous activity (SNA), arterial pressure (AP), and heart rate (HR), and it attenuates the baroreceptor reflex
Ali Aslan et al.
Brain research, 1321, 117-124 (2010-02-02)
The aim of the present study was to investigate the role of NO as a mediator of leptin action at the penicillin-induced epileptiform activity in rat. Thirty minutes after penicillin injection, leptin, at a dose of 1 microg, significantly increased
Luigi Aloe et al.
Neural regeneration research, 9(10), 1025-1030 (2014-09-11)
The purpose of this work was to investigate whether, by intranasal administration, the nerve growth factor bypasses the blood-brain barrier and turns over the spinal cord neurons and if such therapeutic approach could be of value in the treatment of
Articles
Lipid Induced Insulin Resistance
脂肪组织中的胰岛素耐受性减少了循环游离脂肪酸的摄入，并增加了脂肪酶对储存甘油三酯的水解。了解与胰岛素耐受性相关的激素、细胞因子和脂肪因子。
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