L6516
Lymphotactin from mouse
>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym(s):
Lptn
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About This Item
UNSPSC Code:
51111800
MDL number:
biological source
mouse
recombinant
expressed in E. coli
assay
>97% (SDS-PAGE)
form
lyophilized powder
potency
0.05-0.1 mg per mL ED50 (For chemotaxis of BaF/3 hCXR1 cells), 6-12 mg per mL ED50 (for chemotaxis of Il-2 cultured lymphocytes)
mol wt
predicted mol wt ~10 kDa
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
UniProt accession no.
storage temp.
−20°C
Gene Information
mouse ... Xcl1(16963)
General description
Lymphotactin (XCL1) is generated by activated CD8+ T cells, Th1 (T helper)-polarized CD4+ T cells and NK (natural killer) cells. XCR1 (XC chemokine receptor 1) is a lymphotactin receptor.
Biochem/physiol Actions
Lymphotactin (Lptn), a member of the C or γ subfamily of chemokines, is similar to members of the CC and CXC chemokine families, but lacks two of the four cysteine residues. Recombinant mouse lymphotactin has chemotactic activity for lymphocytes, NK cells, and mouse splenocytes, but not for monocytes or neutrophils.
Lymphotactin (XCL1) participates in the formation of lung granuloma. In humans, HIV (human immunodeficiency virus)-1 protein, Tat, upregulates XCL1 expression. Lymphotactin might be involved in the CNS (central nervous system) infiltration of T lymphocytes, leading to HIV-1-mediated neurological diseases.
Member of the C or γ subfamily of chemokines, is similar to members of the CC and CXC chemokine families, but lacks two of the four cysteine residues; has chemotactic activity for lymphocytes, NK cells, and mouse splenocytes, but not for monocytes or neutrophils.
Physical form
Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 1.25 mg bovine serum albumin.
Analysis Note
The biological activity is measured by its ability to chemoattract 3 week IL-2 cultured human lymphocytes and mouse BaF/3 cells transfected with hXCR1.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Yu Lei et al.
The Journal of experimental medicine, 208(2), 383-394 (2011-02-09)
Dendritic cells (DCs) in the thymus (tDCs) are predominantly accumulated in the medulla and contribute to the establishment of self-tolerance. However, how the medullary accumulation of tDCs is regulated and involved in self-tolerance is unclear. We show that the chemokine
Byung Oh Kim et al.
Journal of immunology (Baltimore, Md. : 1950), 172(3), 1888-1895 (2004-01-22)
HIV-1 Tat has been proposed as a key agent in many AIDS-related disorders, including HIV-1-associated neurological diseases. We have recently shown that Tat expression induces a significant increase in T lymphocytes in the brains of Tat transgenic mice. The CNS
Adrian G Rosas-Taraco et al.
American journal of respiratory cell and molecular biology, 41(2), 136-145 (2008-12-23)
Mice infected for 60 days with Mycobacterium tuberculosis were treated with aerosolized XCL1-targeting small interfering RNA (siRNA) to induce local and transient suppression of XCL1/lymphotactin (an important chemokine in tuberculoid granuloma formation). The local pulmonary siRNA therapy resulted in a
J A Hedrick et al.
Journal of immunology (Baltimore, Md. : 1950), 158(4), 1533-1540 (1997-02-15)
Injection of lymphotactin (Lptn) into the peritoneum caused an influx of lymphocytes at 24 h. Phenotypic analysis of the cellular influx showed that a large proportion of these cells were T lymphocytes; however, a large number of NK cells was
G S Kelner et al.
Science (New York, N.Y.), 266(5189), 1395-1399 (1994-11-25)
In this study, the cytokine-producing profile of progenitor T cells (pro-T cells) was determined. During screening of a complementary DNA library generated from activated mouse pro-T cells, a cytokine designated lymphotactin was discovered. Lymphotactin is similar to members of both
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