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L8525

Sigma-Aldrich

Lipase from Candida rugosa

lyophilized powder, ≥40,000 units/mg protein

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Synonym(s):
Triacylglycerol acylhydrolase, Triacylglycerol lipase
CAS Number:
9001-62-1
Enzyme Commission number:
3.1.1.3 (BRENDA, IUBMB)
EC Number:
232-619-9
MDL number:
MFCD00131509
NACRES:
NA.54

biological source

fungus (Candida rugosa)

Quality Level

200

form

lyophilized powder

specific activity

≥40,000 units/mg protein

composition

Protein, ≥4.0%

foreign activity

α-amylase ≤0.05 unit/mg solid
protease ≤0.001 units/mg solid

storage temp.

2-8°C

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

InChI key

QWZUIMCIEOCSJF-CHHCPSLASA-N

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General description

Microbes of plant and animal basis mainly produce lipase enzyme. It can also be produced by marine organisms.

Application

Lipase from C. rugosa is used to catalyze the synthesis of flavor esters such as pentyl propanoate and butyl butanoate used in food enhancement.
Lipase from Candida rugosa has been used in a study to assess the influence of phase segregation on enzyme hydrolytic activity.
Lipase from Candida rugosa has been used:
  • in the hydrolysis of loaded polycaprolactone–20% tricalcium phosphate (PCL–TCP) composites
  • in adsorption of proteins in emulsan/alginate beads and in enzyme assays
  • to catalyse methanolysis and hydrolysis reactions

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Biochem/physiol Actions

Lipases are used in detergent, food, leather, pharmaceutical and diary industries. It has several characteristics like substrate specificity, stereospecificity and regioselectivity. It also participates in various bioconversion reactions like, hydrolysis, esterification, acidolysis and aminolysis.
Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Unit Definition

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.2 at 37 °C using olive oil (30 minute incubation).

Analysis Note

Protein determined by biuret.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Marine fungal and bacterial isolates for lipase production: A comparative study
Advances in Food and Nutrition Research, 78, 71-94 (2016)
Studies on the specificity of Candida rugosa lipase catalyzed esterification reactions in organic media.
BEZBRADICA, D.
J. Serb. Chem. Soc., 71, 31?41-31?41 (2006)
Mariangela Bellusci et al.
Biomacromolecules, 13(3), 805-813 (2012-02-03)
Microbial lipase from Candida rugosa was immobilized by physical adsorption onto an ethylene-vinyl alcohol polymer (EVAL) functionalized with acyl chlorides. To evaluate the influence of the reagent chain-length on the amount and activity of immobilized lipase, three differently long aliphatic
Daniele Zappi et al.
Talanta, 194, 26-31 (2019-01-06)
A newly modified electrode based on glassy carbon (GC) has been prepared and characterized electrochemically for application in electroanalytical chemistry. In particular, a GC screen-printed electrode (SPE) has been modified with nanostructures, namely multi-walled carbon nanotubes (MWCNTs), and TiO2 nanoparticles
An in vitro evaluation of PCL-TCP composites as delivery systems for platelet-rich plasma
Rai B, et al.
Journal of Controlled Release : Official Journal of the Controlled Release Society, 107(2), 330-342 (2005)
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