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Merck
CN

L8525

Lipase from Candida rugosa

lyophilized powder, ≥40,000 units/mg protein

Synonym(s):

Triacylglycerol acylhydrolase, Triacylglycerol lipase

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About This Item

CAS Number:
9001-62-1
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-619-9
EC Number:
3.1.1.3 (BRENDA, IUBMB)
MDL number:
MFCD00131509
Specific activity:
≥40,000 units/mg protein
Biological source:
fungus (Candida rugosa)

Key Documents

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InChI key

QWZUIMCIEOCSJF-CHHCPSLASA-N

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

biological source

fungus (Candida rugosa)

form

lyophilized powder

specific activity

≥40,000 units/mg protein

composition

Protein, ≥4.0%

foreign activity

α-amylase ≤0.05 unit/mg solid, protease ≤0.001 units/mg solid

storage temp.

2-8°C

Quality Level

200

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General description

Microbes of plant and animal basis mainly produce lipase enzyme. It can also be produced by marine organisms.

Application

Lipase from C. rugosa is used to catalyze the synthesis of flavor esters such as pentyl propanoate and butyl butanoate used in food enhancement.
Lipase from Candida rugosa has been used in a study to assess the influence of phase segregation on enzyme hydrolytic activity.
Lipase from Candida rugosa has been used:
  • in the hydrolysis of loaded polycaprolactone–20% tricalcium phosphate (PCL–TCP) composites
  • in adsorption of proteins in emulsan/alginate beads and in enzyme assays
  • to catalyse methanolysis and hydrolysis reactions

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Biochem/physiol Actions

Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Lipases are used in detergent, food, leather, pharmaceutical and diary industries. It has several characteristics like substrate specificity, stereospecificity and regioselectivity. It also participates in various bioconversion reactions like, hydrolysis, esterification, acidolysis and aminolysis.
Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Analysis Note

Protein determined by biuret.

Other Notes

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.2 at 37 °C using olive oil (30 minute incubation).

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

常规特殊物品
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Certificates of Analysis (COA)

Lot/Batch Number
0000461116
0000461116
0000276147
0000276147
SLCM6015

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Daniele Zappi et al.
Talanta, 194, 26-31 (2019-01-06)
A newly modified electrode based on glassy carbon (GC) has been prepared and characterized electrochemically for application in electroanalytical chemistry. In particular, a GC screen-printed electrode (SPE) has been modified with nanostructures, namely multi-walled carbon nanotubes (MWCNTs), and TiO2 nanoparticles
Marine fungal and bacterial isolates for lipase production: A comparative study
Advances in Food and Nutrition Research, 78, 71-94 (2016)
Lipase: An Industrial Enzyme Through Metagenomics (2018)
Enzymatic transesterification monitored by an easy-to-use Fourier transform infrared spectroscopy method
Natalello A, et al.
Biotechnology Journal, 8(1), 133-138 (2013)
Triggered release of proteins from emulsan-alginate beads
Castro G R, et al.
Journal of Controlled Release : Official Journal of the Controlled Release Society, 109(1-3), 149-157 (2005)
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