L9132
Lectin from Phaseolus vulgaris (red kidney bean)
Phytohemagglutinin PHA-P, suitable for cell culture, lyophilized powder
Synonym(s):
Phaseolus vulgaris agglutinin, PHA
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About This Item
EC Number:
232-718-7
UNSPSC Code:
12352202
form
lyophilized powder
potency
≤16 μg per mL agglutination activity (with erythrocytes), ≤10 μg per mL mitogenic activity
technique(s)
cell culture | mammalian: suitable
impurities
salt, free
solubility
PBS: 5 mg/mL
storage temp.
2-8°C
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Biochem/physiol Actions
PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.
Analysis Note
Agglutination activity is expressed in mg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8 after 1 hr incubation at 25°C. Mitogenic activity is determined by BrdU incorporation in HeLa cell cultures.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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