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Merck
CN

L9382

Lactic Dehydrogenase from porcine heart

Type VII-S, ammonium sulfate suspension, ~500 units/mg protein

Synonym(s):

(S)-Lactate: NAD+ oxidoreductase, L-LDH, L-Lactic dehydrogenase LDH-1 (H4) Isoenzyme from Porcine Heart, LAD, LD, Lactate Dehydrogenase from porcine heart

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About This Item

CAS Number:
UNSPSC Code:
12352204
EC Number:
232-617-8
EC Number:
Technical Service
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type

Type VII-S

form

ammonium sulfate suspension

specific activity

~500 units/mg protein

isotype

LDH1 (H4

foreign activity

glutamic-pyruvic transaminase <0.03%, pyruvate kinase, myokinase, malic dehydrogenase, and glutamic-oxalacetic transaminase <0.01%

storage temp.

2-8°C

Biochem/physiol Actions

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Physical form

Crystalline suspension in 3.2 M (NH4)2SO4 solution, pH 6.0

Analysis Note

Protein determined by biuret.

Other Notes

One unit will reduce 1.0μmole of pyruvate to L-lactate per min at pH7.5 at 37 °C.

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Deshdeepak Sahni et al.
Journal of neurosurgery. Pediatrics, 11(5), 575-583 (2013-03-12)
Graphene possesses unique electrical, physical, and chemical properties that may offer significant potential as a bioscaffold for neuronal regeneration after spinal cord injury. The purpose of this investigation was to establish the in vitro biocompatibility of pristine graphene for interface
D Steffens et al.
Journal of biomedical nanotechnology, 9(4), 710-718 (2013-04-30)
The association of stem cells (SCs) with biomaterials promises to be the protagonist for future regenerative medicine in the treatment of tissue and organ lesions. Stem cells were cultivated in scaffolds constructed by the electrospinning technique, using poly-D,L-lactic acid (PDLLA)
Minh Vu Chuong Nguyen et al.
Biochemical pharmacology, 85(11), 1644-1654 (2013-04-16)
NADPH oxidase Nox4 is expressed in a wide range of tissues and plays a role in cellular signaling by providing reactive oxygen species (ROS) as intracellular messengers. Nox4 oxidase activity is thought to be constitutive and regulated at the transcriptional
Wei Quan et al.
Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan, 33(1), 85-91 (2013-04-20)
To determine the cardioprotective effect of magnesium lithospermate B (MLB) on myocardial ischemia/reperfusion (MI/R) injury and to investigate the antioxidant potential in vivo and in vitro. MI/R injury was induced by the occlusion of left anterior descending coronary artery for
Serum lactate dehydrogenase as an early marker of posterior reversible encephalopathy syndrome: keep your eyes open on the score of severity brain oedema.
M Vargas et al.
Anaesthesia and intensive care, 41(3), 436-437 (2013-05-11)

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