L9514
Ricin A chain from Ricinus communis (castor bean)
buffered aqueous glycerol solution
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About This Item
Form:
buffered aqueous glycerol solution
Storage temp.:
2-8°C
form
buffered aqueous glycerol solution
Quality Segment
potency
≥250 μg/mL per mL agglutination activity
composition
Protein, ≥0.3 mg/mL Lowry-TCA
storage temp.
2-8°C
Biochem/physiol Actions
Ricin A chain inactivates the 60 S ribosomal subunit of eukaryotic cells, inhibiting protein synthesis. This toxic activity is independent of the presence of the B chain of ricin.
Physical form
Solution in 40% glycerol containing 10 mM phosphate, pH 6.0, 0.15 M NaCl, 10 mM galactose and 0.5 mM dithioerythritol
Preparation Note
Purified by affinity chromatography.
Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 7.2, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hour incubation at 25°C.
Electrophoretically pure (SDS-PAGE)
Other Notes
Ricinus communis toxin (also named RCA60, RCAII, Ricin D, or RCL III) is a highly toxic protein, mol. wt. ~60 kDa, composed of two chains, A and B, connected by a single disulfide bridge.
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 2 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Skin Sens. 1
Storage Class
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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