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LGC602102

BAC-Optimized Replicator v2.0 Electrocompetent Cells, (DUOs)

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pkg of 24 reactions (12 x 50 µL), Cfu/µg ≥1 x 1010

Synonym(s):

BAC libraries cells

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About This Item

UNSPSC Code:
41106202

biological source

Escherichia coli

manufacturer/tradename

Lucigen 60210-2

packaging

pkg of 24 reactions (12 x 50 µL)

growth mode

growth or suspension

technique(s)

microbiological culture: suitable

cell transformation

competent cell type: electrocompetent
transformation efficiency: 1 x 1010 cfu/μg

shipped in

dry ice

storage temp.

-65 to -96°C

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General description

BAC-Optimised Replicator™ v2.0 Electrocompetent Cells are designed for BAC library construction or trasformation of large constructs. These cells are required for on-demand, copy number amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight® Kits, thereby increasing DNA yields. BAC-Optimised Replicator Cells are components of Biosearch Technologies Cloning Kits. Packed as DUOs (2 transformations per tube)

Application

  • Cloning
  • BAC Library construction
  • Cloning of large constructs

Features and Benefits

  • Highest available transformation efficiencies for library construction and screening
  • Ideal for BAC library construction or transformation of large inserts
  • Optimised for high yields of DNA
Transformation efficiency: ≥ 1 x 1010 (cfu/µg pUC or pKanR DNA))

Genotype BAC-Optimized Replicator v2.0: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galUgalK rpsL nupG (attL araC-PBAD-trfA250 bla attR) λ-. Note that Replicator v2.0 cells are AmpR (bla gene) (StrR)

Components:


  • BAC-Optimized Replicator v2.0 Electrocompetent Cells
  • Transformation Control pKanR
  • Arabinose Induction Solution
  • Recovery Media

Legal Information

CopyRight is a registered trademark of Lucigen Corporation
Replicator is a trademark of Lucigen Corporation
pEZ is a trademark of Lucigen Corporation
pSMART is a registered trademark of Lucigen Corporation

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