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About This Item
UNSPSC Code:
41106202
Biological source:
Escherichia coli
Growth mode:
growth or suspension
Manufacturer/tradename:
LGC 60242-2
biological source
Escherichia coli
manufacturer/tradename
LGC 60242-2
packaging
pkg of 24 reactions (12 x 50 µL)
growth mode
growth or suspension
technique(s)
microbiological culture: suitable
cell transformation
competent cell type: electrocompetent
transformation efficiency: 1 x 1010 cfu/μg
shipped in
dry ice
storage temp.
-65 to -96°C
General description
Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be propagated stably.
Application
- Cloning and maintaining unstable or repetitive DNA sequences.
- Recommended for high efficiency propagation of GeCKO CRISPR libraries (1)
- Ideal for single-guide RNA lentiviral library construction (2)
Features and Benefits
- Clone repetitive sequences and lentiviral libraries
- Stabilize direct repeats and create lentiviral constructs
- Transformation efficiency: ≥ 1 x 1010 (cfu/µg)
- Genotype: Endura: recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ– leu mtl-1 F– mcrB mrr hsdS20(rB–, mB–) Components: Endura Electrocompetent Cells; pUC 19 trasformation control; recovery media
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| LGC602422-1KIT | 04065273337747 |