BigEasy^® v2.0 Linear Cloning Kit (pJAZZ^®-OC NotI Vector)

The BigEasy^® Kit with the pJAZZ^® vector is ideal for constructing bias-free, large-insert genomic libraries, or for cloning difficult DNA of any size up to 30 kb. Because the novel pJAZZ vector is maintained as a linear molecule, the ends of the vector can rotate freely. As a result, the vector does not supercoil. Without the torsional stress induced by supercoiling, otherwise unclonable sequences (e.g., repetitive, or A/T-rich or G/C-rich sequences) are stabilized. The pJAZZ linear cloning vector is maintained at low copy number (5-10/cell) in BigEasy^®-TSA^™ Electrocompetent Cells (>4 × 10¹⁰ cfu/μ g), which are required for transformation and propagation. The copy number can be induced 5-10X in this strain. In addition, the pJAZZ vector incorporates Lucigen′s CloneSmart^® technology for transcription-free cloning, which further increases insert stability. pJAZZ clones can be isolated using conventional plasmid prep methods and sequenced using standard techniques.

BigEasy is a registered trademark of Lucigen Corporation

CloneSmart is a registered trademark of Lucigen Corporation

TSA is a trademark of Lucigen Corporation

pJAZZ is a registered trademark of Lucigen Corporation