M1194
Anti-MTA2/MTA1L1 (RT-16) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Synonym(s):
Anti-Metastasis-associated Gene
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About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IP, WB
Citations:
5
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 73 kDa
species reactivity
human, rat, mouse
technique(s)
immunoprecipitation (IP): 1-2 μL using 293-T cell lysate, indirect immunofluorescence: 1:100-1:200 using methanol-acetone fixed C2 myoblasts, western blot: 1:2,000-1:4,000 using nuclear extracts of MCF7 cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... MTA2(9219)
mouse ... Mta2(23942)
rat ... Mta2(361724)
General description
Metastasis-associated protein (MTA2) comprises bromo-adjacent homology (BAH), Egl-27 and MTA1 homology 2 (ELM2), Swi3, Ada2, N-Cor, and TFIIIB (SANT), and an atypical GATA zinc finger domain. It is a phosphorylated protein. MTA2 gene is mapped to human chromosome 11q12.3. Human MTA2 shares approximately 80% overall homology to human MTA1 and MTA3 proteins, with the C-terminus being more divergent than the N-terminus.
Immunogen
synthetic peptide corresponding to amino acids 626-641 of human MTA2/MTA1L1, conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide is conserved in human, mouse, and rat, and is not present in other members of the family.
Application
Anti-MTA2/MTA1L1 (RT-16) antibody produced in rabbit has been used in:
- tandem immunoprecipitation
- immunoblotting
- immunofluorescence
Biochem/physiol Actions
Anti MTA2/MTA1L1 (RT-16) specifically recognizes MTA2/MTA1L1.
Metastasis-associated protein (MTA2) mediates cytoskeletal reorganization. It interacts with the histone deacetylases (HDAC) within the nuclear remodeling and deacetylation complexes namely, nucleosome remodeling and deacetylase (Mi2/NuRD). This interaction mediates transcriptional repression. Also called p53 target protein in deacetylase complex (PID), it regulates p53 function and represses p53-dependent transcriptional activation, growth arrest and apoptosis. MTA2 also favors tumor growth and migration.
Physical form
Solution in 0.01 M phophate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Regulatory Information
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Role of MTA2 in human cancer
Covington KR and Fuqua SAW
Cancer Metastasis Reviews, 921-928 (2014)
TP53 mutation, epithelial-mesenchymal transition, and stemlike features in breast cancer subtypes
Coradini D, et al.
Journal of Biomedicine and Biotechnology (2012)
Weijun Ding et al.
International journal of clinical and experimental pathology, 8(6), 7173-7180 (2015-08-12)
Association of MTA2 expression with presence, development, metastasis and prognosis of colorectal cancer (CRC) was investigated. 90 CRC-related cases with follow-up information were made into tissue microarrays according to the paired principle of cancer tissues and the adjacent tissues. Subsequently
J Luo et al.
Nature, 408(6810), 377-381 (2000-12-01)
The p53 tumour suppressor is a transcriptional factor whose activity is modulated by protein stability and post-translational modifications including acetylation. The mechanism by which acetylated p53 is maintained in vivo remains unclear. Here we show that the deacetylation of p53
Gohei Nishibuchi et al.
The Journal of biological chemistry, 289(42), 28956-28970 (2014-09-06)
Histone H3K4 methylation has been linked to transcriptional activation. KDM5A (also known as RBP2 or JARID1A), a member of the KDM5 protein family, is an H3K4 demethylase, previously implicated in the regulation of transcription and differentiation. Here, we show that
Related Content
Datasheet
Global Trade Item Number
| SKU | GTIN |
|---|---|
| M1194-200UL | 04061835808434 |
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