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Merck
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M218

Monoclonal Anti-Nicotinic Acetylcholine Receptor (α4 Subunit) antibody produced in rat

~1 mg/mL, clone mAb 299, purified immunoglobulin, buffered aqueous solution

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD00677279
Conjugate:
unconjugated
Clone:
mAb 299, monoclonal
Application:
IHC (f), RIA
Citations:
1

Key Documents

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biological source

rat

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

mAb 299, monoclonal

form

buffered aqueous solution

species reactivity

human, chicken, rat

concentration

~1 mg/mL

technique(s)

immunohistochemistry (frozen sections): 1:800, radioimmunoassay: suitable

isotype

IgG1

UniProt accession no.

P43681

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CHRNA4(1137)
rat ... Chrna4(25590)

Immunogen

nicotinic acetylcholine receptor (AchR) from the rat brain.

Biochem/physiol Actions

Binds to native and denatured α4 AchR. May be used to quantitate α4 AchR′s on the cell surface. The epitope is located on the extracellular surface of the α4 subunit of the AchR.

Physical form

Solution in 20 mM Na3PO4, pH 7.2, containing 150 mM NaCl and 0.05% sodium azide.

Legal Information

Sold with permission of the Salk Institute

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
070K47062
070K4706

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V V Pollock et al.
Neuroscience, 158(4), 1311-1325 (2008-12-23)
Neuronal nicotinic receptor alpha4 subunits associated with nicotinic alpha4beta2 receptors are phosphorylated by cyclic AMP-dependent protein kinase (PKA) and protein kinase C (PKC), but the stages of receptor formation during which phosphorylation occurs and the functional consequences of kinase activation

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