M221
Monoclonal Anti-Nicotinic Acetylcholine Receptor (α3 Subunit) antibody produced in rat
~5 mg/mL, clone mAb 313, affinity isolated antibody, buffered aqueous solution
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About This Item
UNSPSC Code:
12352203
MDL number:
biological source
rat
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
mAb 313, monoclonal
form
buffered aqueous solution
species reactivity
chicken
concentration
~5 mg/mL
technique(s)
immunohistochemistry (frozen sections): 1:4,000
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
chicken ... CHRNA3(386578)
Immunogen
recombinant large cytoplasmic domain of the α3 subunit (amino acids 315-441) of the nicotinic acetylcholine receptor from chick.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Biochem/physiol Actions
Binds to native and denatured detergent-solubilized α3 subunit of AchR. Does not bind α1, α4, α5, α7, α8 and β2.
Physical form
Solution in 20 mM Na3PO4, pH 7.2, containing 150 mM NaCl and 0.05% sodium azide.
Legal Information
Sold with permission of the Salk Institute
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Regulatory Information
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P J Whiting et al.
Brain research. Molecular brain research, 10(1), 61-70 (1991-04-01)
Neuronal nicotinic acetylcholine receptors (AChRs) are composed of two types of subunits: ACh-binding (termed alpha 2, alpha 3, alpha 4 ...) and structural (termed beta 2, beta 3, beta 4 ...). AChR subtypes composed of combinations of subunits of these
Lindstrom
Neurotransmissions, 12, 2-2 (1996)
Margarita Villar et al.
Biomolecules, 10(4) (2020-04-09)
Ticks are obligate hematophagous arthropods and vectors of pathogens affecting human and animal health worldwide. Cement is a complex protein polymerization substance secreted by ticks with antimicrobial properties and a possible role in host attachment, sealing the feeding lesion, facilitating
W G Conroy et al.
Neuron, 9(4), 679-691 (1992-10-01)
The acetylcholine receptor (AChR) alpha 5 gene has been classified as a member of the AChR gene family based on sequence homology. Expression studies, however, have yet to identify a function for the alpha 5 gene product or even to
Benjaporn Buranrat et al.
Oncology reports, 34(5), 2790-2796 (2015-09-10)
Ferritin is a major iron storage protein and essential for iron homeostasis. It has a wide range of functions in the body including iron delivery, immunosuppression, angiogenesis, and cell proliferation. Ferritin is overexpressed in many cancer cells, but its precise
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