M252
MIG human
>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
biological source
human
recombinant
expressed in E. coli
Assay
>97% (SDS-PAGE)
form
lyophilized powder
potency
0.2-0.6 μg/mL ED50
mol wt
Mw ~11.7 kDa
storage condition
avoid repeated freeze/thaw cycles (Storage in "frost-free" freezers is not recommended)
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... CXCL9(4283)
Biochem/physiol Actions
MIG, a member of the α (CXC) chemokine family of inflammatory and immunoregulatory cytokines, is a chemoattractant for activated T lymphocytes and TIL, but not for neutrophils or monocytes. The MIG gene is induced in macrophages and primary glial cells of the central nervous system specifically in response to IFN-γ. MIG with large C-terminal deletions have diminished activity in calcium flux assay. The chemokine receptor CXCR3 is specific for MIG and IP-10 and highly expressed in IL-2-activated T-lymphocytes.
Physical form
Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 0.5 mg bovine serum albumin.
Analysis Note
The biological activity is measured by its ability to chemoattract human lymphocytes cultured for 8-10 days in the presence of IL-2.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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F Liao et al.
The Journal of experimental medicine, 182(5), 1301-1314 (1995-11-01)
Mig is a chemokine of the CXC subfamily that was discovered by differential screening of a cDNA library prepared from lymphokine-activated macrophages. The mig gene is inducible in macrophages and in other cells in response to interferon (IFN)-gamma. We have
J M Farber
Biochemical and biophysical research communications, 192(1), 223-230 (1993-04-15)
Characterization of lymphokine-activated genes in mouse macrophages led to the identification previously of Mumig, an interferon-gamma-inducible murine gene that encodes a member of the chemokine family of cytokines. The Mumig cDNA probe was used to screen a cDNA library prepared
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