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Merck
CN

M3274

SAFC

Medium 199

powder, Modified, suitable for cell culture

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About This Item

UNSPSC Code:
12352200
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form

powder

IVD

for in vitro diagnostic use

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

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General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Preparation Note

Formulated to contain 10.9 grams of powder per liter of medium.
Supplement with 0.1 g/L L-glutamine, 0.35 g/L sodium bicarbonate.
With Hanks′ salts. Without L-glutamine, phenol red and sodium bicarbonate.

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