M3274
Medium 199
powder, Modified, suitable for cell culture
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About This Item
UNSPSC Code:
12352200
form
powder
IVD
for in vitro diagnostic use
technique(s)
cell culture | mammalian: suitable
storage temp.
2-8°C
General description
Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.
Preparation Note
Formulated to contain 10.9 grams of powder per liter of medium.
Supplement with 0.1 g/L L-glutamine, 0.35 g/L sodium bicarbonate.
With Hanks′ salts. Without L-glutamine, phenol red and sodium bicarbonate.
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