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Merck
CN

M5427

Sigma-Aldrich

Anti-Matrix Metalloproteinase-9 Hinge Region antibody produced in rabbit

~0.2 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-MMP-9

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About This Item

MDL number:
UNSPSC Code:
12352203
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biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

concentration

~0.2 mg/mL

technique(s)

immunoprecipitation (IP): suitable
indirect ELISA: 0.1-1.0 μg/mL
western blot: 0.5-2 μg/mL using PMA stimulated A431 cells

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... MMP9(4318)

Immunogen

synthetic peptide corresponding to the hinge region (surrounding amino acid 475) of human matrix metalloproteinase-9 (gelatinase-B).

Biochem/physiol Actions

By immunoblotting, the antibody reacts with a major band at 65 kDa and faint band at approx. 92 kDa in a PMA whole cell lysate.

Physical form

Solution in phosphate buffered saline containing 0.05% sodium azide.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Jamie Berger et al.
Neurological research, 29(6), 610-614 (2007-05-31)
High mortality incidence after serious systemic thermal injury is believed to be linked to significant increases in cerebral permeability, ultimately leading to irreversible blood-brain barrier (BBB) breakdown. The aim of this study was to investigate whether disruption of microvascular integrity
Manikantan S Kiran et al.
Vascular cell, 3(1), 6-6 (2011-02-26)
The mechanism of cell-cell contact dependent regulation of pericellular proteolysis in angiogenesis was examined by studying the expression of MMPs using isolated HUVECs in culture. Zymography, Immunoblot and RT-PCR analysis showed that the production and secretion of matrixmetalloproteinase-2 and matrixmetalloproteinase-9

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