M5539
Anti-Macrophage Inflammatory Protein-2 antibody produced in goat
affinity isolated antibody, lyophilized powder
Synonym(s):
Anti-MIP-2
biological source
goat
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized powder
species reactivity
mouse
technique(s)
indirect ELISA: suitable, neutralization: suitable, western blot: suitable
UniProt accession no.
storage temp.
−20°C
Gene Information
mouse ... Cxcl2(20310)
General description
Macrophage Inflammatory Protein-2 belongs to the C-X-C family of chemokines and acts as chemoattractant for neutrophils. MIP-2 was originally identified as heparin-binding protein secreted in response to endotoxin. This chemokine acts majorly to induce influx of neutrophils following infection by pneumococci, hypersensitive reaction to viral antigen and inflammation due to glomerular nephritis. Pulmonary introduction of LPS results in rapid increase in the levels of MIP-2 producing inflammation. Anti-Macrophage Inflammatory Protein-2 antibody is specific for mouse MIP-2.
Immunogen
recombinant mouse MIP-2 expressed in E. coli.
Application
Anti-Macrophage Inflammatory Protein-2 antibody may be used for ELISA at a working antibody concentration of 0.5-1.0 μg/ml. For immunoblotting, antibody concentration of 0.1-0.2 μg/ml is recommended. The antibody is suitable for neutralization reactions.
Biochem/physiol Actions
The antibody shows <5% cross-reactivity with recombinant human GROα, recombinant human GROβ and recombinant human GROγ.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.
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Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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A B Lentsch et al.
Hepatology (Baltimore, Md.), 27(4), 1172-1177 (1998-04-16)
Hepatic injury induced by ischemia and reperfusion is an important clinical problem after liver resection or transplantation. Neutrophils are known to mediate the organ injury, but the precise mechanisms leading to hepatic neutrophil recruitment are undefined. Two CXC chemokines, macrophage
Terrence M Tumpey et al.
Journal of virology, 76(16), 8050-8057 (2002-07-23)
BALB/c mice sensitized to herpes simplex virus type 1 (HSV-1) develop a vigorous delayed-type hypersensitivity (DTH) response upon intradermal virus antigen challenge. Although CD4(+) T cells are a key mediator of this response, neutrophils are the most abundant cells at
John B Zamjahn et al.
American journal of physiology. Lung cellular and molecular physiology, 301(4), L568-L574 (2011-07-12)
Previously we showed that cytokine-induced neutrophil chemoattractant (CINC), but not macrophage inflammatory protein-2 (MIP-2), is detected in plasma after intratracheal challenge with LPS or the particular chemokines. To further understand the differences between CINC and MIP-2 flux from the lung
L Feng et al.
The Journal of clinical investigation, 95(3), 1009-1017 (1995-03-01)
The role of the chemokine, macrophage inflammatory protein-2 (MIP-2), during anti-glomerular basement membrane (GBM) antibody (Ab) glomerulonephritis (GN) was studied. Rat MIP-2 cDNA had been cloned previously. Recombinant rat MIP-2 (rMIP-2) from Escherichia coli exhibited neutrophil chemotactic activity and produced
S D Wolpe et al.
Proceedings of the National Academy of Sciences of the United States of America, 86(2), 612-616 (1989-01-01)
In response to endotoxin, macrophages secrete a protein with a molecular mass of approximately 6000 Da and with an affinity for heparin. This protein, which we term "macrophage inflammatory protein 2," is a potent chemotactic agent for human polymorphonuclear leukocytes.
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