M5813
Mimetic Orange 2 Ligand Affinity Adsorbent
suspension
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About This Item
UNSPSC Code:
23151817
MDL number:
form
suspension
shipped in
wet ice
storage temp.
2-8°C
Application
Ligand affinity adsorbents are used in protein chromatography, affinity chromatography and dye resins. Ligand affinity adsorbents have been used to describe a simple protocol for the purification of Taq Polymerase from E. coli lysates as well as for the purification of other DNA polymerases.
signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
Storage Class
3 - Flammable liquids
wgk
WGK 1
flash_point_f
95.0 °F - closed cup
flash_point_c
35 °C - closed cup
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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Crystal structure of pentaerythritol tetranitrate reductase: "flipped" binding geometries for steroid substrates in different redox states of the enzyme.
Barna, T.M., et al.
Journal of Molecular Biology, 310(2), 443-447 (2001)
Huma Khan et al.
The FEBS journal, 272(18), 4660-4671 (2005-09-15)
The roles of His181, His184 and Tyr186 in PETN reductase have been examined by mutagenesis, spectroscopic and stopped-flow kinetics, and by determination of crystallographic structures for the Y186F PETN reductase and reduced wild-type enzyme-progesterone complex. Residues His181 and His184 are
Huma Khan et al.
The Journal of biological chemistry, 277(24), 21906-21912 (2002-03-30)
The reaction of pentaerythritol tetranitrate reductase with reducing and oxidizing substrates has been studied by stopped-flow spectrophotometry, redox potentiometry, and X-ray crystallography. We show in the reductive half-reaction of pentaerythritol tetranitrate (PETN) reductase that NADPH binds to form an enzyme-NADPH
P R Binks et al.
Applied and environmental microbiology, 62(4), 1214-1219 (1996-04-01)
A mixed microbial culture capable of metabolizing the explosive pentaerythritol tetranitrate (PETN) was obtained from soil enrichments under aerobic and nitrogen-limiting conditions. A strain of Enterobacter cloacae, designated PB2, was isolated from this culture and was found to use PETN
Huma Khan et al.
The Journal of biological chemistry, 279(29), 30563-30572 (2004-05-07)
The structure of pentaerythritol tetranitrate (PETN) reductase in complex with the nitroaromatic substrate picric acid determined previously at 1.55 A resolution indicated additional electron density between the indole ring of residue Trp-102 and the nitro group at C-6 of picrate.
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