M5948
Anti-MIB1 (C-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-DAPK-interacting protein 1, Anti-DIP-1, Anti-KIAA1323, Anti-Mindbomb homolog 1, Anti-Ubiquitin ligase mind bomb
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~110 kDa
species reactivity
rat, mouse, human
concentration
~1.0 mg/mL
technique(s)
western blot: 0.5-1 μg/mL using lysates of A549 or PC12 cells.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... MIB1(57534)
mouse ... Mib1(225164)
rat ... Mib1(307594)
General description
MIB1 (Mindbomb homology 1) are E3 ubiquitin ligases. It is abundantly expressed in both embryos and adult tissues, whereas MIB2 is highly expressed in adult tissues.
Mindbomb homolog 1 (MIB1) is an ubiquitin ligase which possesses two substrate recognition domains, ankyrin repeats and an amino terminal zinc finger domain.
Immunogen
synthetic peptide corresponding to amino acids 924-939 of human MIB1, conjugated to KLH. The corresponding sequence is identical in rat and mouse.
Application
Anti-MIB1 (C-terminal) antibody produced in rabbit has been used in:immunofluorescence, immunoprecipitation , western blotting immunohistochemistry
Anti-MIB1 (N-terminal) antibody produced in rabbit has been used for immunofluorescence.
Biochem/physiol Actions
MIB1 is critical for mammalian development and cellular differentiation. It also plays a role in neuronal morphogenesis partially through physical interaction with cyclin-dependent kinase 5 (CDK5)/p35. Knockout of MIB1 in mice results in embryonic mortality with many defects observed in somitogenesis, neurogenesis, vasculogenesis and cardiogenesis.
Mindbomb homolog 1 (MIB1) aids in the ubiquitination and internalization of Notch ligands such as the protein δ. It also acts as a positive regulator in the activation of nuclear factor-κB (NF-κB).
Physical form
Solution in 0.01 M phosphate buffered saline, pH7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Mindbomb 1, an E3 ubiquitin ligase, forms a complex with RYK to activate Wntbeta-catenin signaling
Berndt JD, et al.
The Journal of Cell Biology, 194(5), 737-750 (2011)
Neuronal morphogenesis is regulated by the interplay between cyclin-dependent kinase 5 and the ubiquitin ligase mind bomb 1
Choe EA, et al.
The Journal of Neuroscience, 27(35), 9503-9512 (2007)
Differential routing of mindbomb1 via centriolar satellites regulates asymmetric divisions of neural progenitors
Tozer S, et al.
Neuron, 93(3), 542-551 (2017)
Clotilde C N Renaud et al.
Communications biology, 6(1), 1271-1271 (2023-12-16)
Centriolar satellites are multiprotein aggregates that orbit the centrosome and govern centrosome homeostasis and primary cilia formation. In contrast to the scaffold PCM1, which nucleates centriolar satellites and has been linked to microtubule dynamics, autophagy, and intracellular trafficking, the functions
Orhi Barroso-Gomila et al.
Nature communications, 14(1), 7656-7656 (2023-11-24)
Hundreds of E3 ligases play a critical role in recognizing specific substrates for modification by ubiquitin (Ub). Separating genuine targets of E3s from E3-interactors remains a challenge. We present BioE3, a powerful approach for matching substrates to Ub E3 ligases
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