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About This Item
UNSPSC Code:
12352200
storage temp.
2-8°C
Application
SDS protein separation medium is a buffered replaceable matrix designed for high resolution separation of SDS-denatured proteins by capillary electrophoresis. Separation of proteins by molecular size capillary electrophoresis requires a sieving matrix in the capillary column. Cross-linked polyacrylamide-filled capillaries have been used for SDS-PAGE of proteins, but the lifetimes of the columns were generally short. More recently, UV-transparent replaceable matrices have been used in which the solution is removed form the capillary tube by pressure and replaced with fresh sieving solution between each run.
Other Notes
Contains sieving matrix in Tris buffer and SDS.
signalword
Danger
Hazard Classifications
Acute Tox. 2 Oral - Carc. 2 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
Regulatory Information
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Instructions
A S Cohen et al.
Journal of chromatography, 397, 409-417 (1987-06-26)
High-performance capillary sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) has been developed for the separation and molecular weight (MW) determination of peptides and proteins. In this work, acrylamide was polymerized in fused-silica capillaries of 75 microns I.D. and 10 or
High performance capillary electrophoresis of SDS-protein complexes using UV-transparent polymer networks.
Ganzler, K., et al.
Analytical Chemistry, 64, 2655-2655 (1992)
Capillary electrophoresis with polymer matrices: DNA and protein separation and analysis.
B L Karger et al.
Methods in enzymology, 271, 293-319 (1996-01-01)

