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M7154

2-Mercaptoethanol

suitable for electrophoresis

Synonym(s):

β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol

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About This Item

Linear Formula:
HSCH2CH2OH
CAS Number:
60-24-2
Molecular Weight:
78.13
EC Number:
200-464-6
UNSPSC Code:
12352200
PubChem Substance ID:
24897143
Beilstein/REAXYS Number:
773648
MDL number:
MFCD00004890
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vapor density

2.69 (vs air)

vapor pressure

1 mmHg ( 20 °C)

assay

>99% (GC)

expl. lim.

18 %

concentration

14.3 M (pure liquid)

technique(s)

electrophoresis: suitable

refractive index

n20/D 1.500 (lit.)

bp

157 °C (lit.)

solubility

water: soluble

density

1.114 g/mL at 25 °C (lit.)

cation traces

Cu: <1 ppm, Fe: <1 ppm, Pb: <5 ppm, Zn: <1 ppm

SMILES string

OCCS

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

InChI key

DGVVWUTYPXICAM-UHFFFAOYSA-N

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Other Notes

This product has been replaced by M3148-SIGMA | 2-Mercaptoethanol for molecular biology, for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

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Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

target_organs

Liver,Heart

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

165.2 °F - closed cup

flash_point_c

74 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Regulatory Information

新产品

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Certificates of Analysis (COA)

Lot/Batch Number
SHBB0964V
SHBB0965V
84796LM
67096CM
36696LK

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