M7527
Myoglobin from sperm whale skeletal muscle
recombinant, expressed in E. coli, 95-100%, buffered aqueous solution
recombinant
expressed in E. coli
Quality Level
Assay
95-100%
form
buffered aqueous solution
Iron content
~0.30%
UniProt accession no.
storage temp.
−20°C
Gene Information
Physeter macrocephalus ... MB(102975021)
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Physical form
Solution in 0.02 M Tris HCl, pH 8.0
Other Notes
Contains an N-terminal methionine not present in the natural product. Amino acid sequence not confirmed by Sigma.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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B A Springer et al.
Proceedings of the National Academy of Sciences of the United States of America, 84(24), 8961-8965 (1987-12-01)
Sperm whale myoglobin was expressed in Escherichia coli from a totally synthetic gene inserted in the expression vector pUC19. The gene was constructed as 23 overlapping oligonucleotides encoding both strands of the DNA. Gene synthesis provides several advantages over traditional
Chromatograms
application for HPLC
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