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Merck
CN

M7819

Sigma-Aldrich

β-Mannosidase from Helix pomatia

BioReagent, Proteomics Grade

Synonym(s):

β-D-Mannoside mannohydrolase

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About This Item

CAS Number:
9025-43-8
EC Number:
3.2.1.25 (BRENDA, IUBMB)
MDL number:
MFCD00130638
UNSPSC Code:
12352204

Key Documents

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biological source

Helix pomatia

product line

BioReagent

Assay

≥90% (SDS-PAGE)

form

lyophilized solid

quality

Proteomics Grade

mol wt

94 kDa

storage temp.

−20°C

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Packaging

Provided with 5× concentrate reaction buffer

Physical form

Enzyme is lyophilized from 10 mM sodium acetate buffer, pH 4.0, containing BSA and sodium chloride.

Other Notes

One unit will hydrolyze 1 μmole of p-nitrophenyl β-D-mannopyranoside to p-nitrophenol (measured at 400 nm) and D-mannose per minute at pH 4.0 at 37 °C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Thi Huynh et al.
BMC genomics, 12 Suppl 3, S22-S22 (2012-03-06)
Lysosomal β-D-mannosidase is a glycosyl hydrolase that breaks down the glycosidic bonds at the non-reducing end of N-linked glycoproteins. Hence, it is a crucial enzyme in polysaccharide degradation pathway. Mutations in the MANBA gene that codes for lysosomal β-mannosidase, result
A P Félix et al.
Journal of animal science, 90(9), 3060-3067 (2012-05-16)
This experiment aimed at evaluating the effects of including the enzyme, β-mannanase, in dog (Canis lupus familiaris) diets based on either poultry (Gallus gallus domesticus) by-product meal (PBM) or soybean [Glycine max (L.) Merr.] Meal (SBM). The second objective was
Eleni Papakonstantinou et al.
American journal of respiratory cell and molecular biology, 47(6), 759-767 (2012-08-07)
Glycosaminoglycans (GAGs), especially hyaluronic acid (HA), regulate tissue flexibility, cell motility, and inflammation. Airway smooth muscle cells (ASMCs) of patients with asthma exhibit abnormal HA metabolism, which contributes to inflammation and remodeling. Here, we investigated the effects of glucocorticoids and
Kimihiko Mizutani et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 68(Pt 10), 1164-1168 (2012-10-03)
β-1,4-Mannanase (EC 3.2.1.78) catalyzes the hydrolysis of β-1,4-glycosidic bonds within mannan, a major constituent group of the hemicelluloses. Bivalves and gastropods possess β-1,4-mannanase and may degrade mannan in seaweed and/or phytoplankton to obtain carbon and energy using the secreted enzymes
Jia Zheng et al.
Bioresource technology, 118, 257-264 (2012-06-19)
An industrial medium, Corn Steep Liquor Powder Dextrose (CSD medium) was developed for constitutive expression of recombinant β-mananase by Pichia pastoris. The β-mananase activity (513 U/mL) with CSD medium was 1.64- and 2.5-fold higher than with YPD and BSM in
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