M7819
β-Mannosidase from Helix pomatia
BioReagent, Proteomics Grade
Synonym(s):
β-D-Mannoside mannohydrolase
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About This Item
Assay:
≥90% (SDS-PAGE)
Biological source:
Helix pomatia
biological source
Helix pomatia
product line
BioReagent
assay
≥90% (SDS-PAGE)
form
lyophilized solid
quality
Proteomics Grade
mol wt
94 kDa
storage temp.
−20°C
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Packaging
Provided with 5× concentrate reaction buffer
Physical form
Enzyme is lyophilized from 10 mM sodium acetate buffer, pH 4.0, containing BSA and sodium chloride.
Other Notes
One unit will hydrolyze 1 μmole of p-nitrophenyl β-D-mannopyranoside to p-nitrophenol (measured at 400 nm) and D-mannose per minute at pH 4.0 at 37 °C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Jia Zheng et al.
Bioresource technology, 118, 257-264 (2012-06-19)
An industrial medium, Corn Steep Liquor Powder Dextrose (CSD medium) was developed for constitutive expression of recombinant β-mananase by Pichia pastoris. The β-mananase activity (513 U/mL) with CSD medium was 1.64- and 2.5-fold higher than with YPD and BSM in
Guojie Jin et al.
Bioresource technology, 111, 378-382 (2012-03-01)
Lipids produced by oleaginous microorganisms are a potential feedstock for biodiesel production and chemical synthesis. Yet, the costs of microbial lipids remain high, partially because the lipid recovery process is tedious and costly. In the present study, enzyme-assisted extraction of
A P Félix et al.
Journal of animal science, 90(9), 3060-3067 (2012-05-16)
This experiment aimed at evaluating the effects of including the enzyme, β-mannanase, in dog (Canis lupus familiaris) diets based on either poultry (Gallus gallus domesticus) by-product meal (PBM) or soybean [Glycine max (L.) Merr.] Meal (SBM). The second objective was
Yuya Kumagai et al.
Biochimie, 94(12), 2783-2790 (2012-09-27)
Mannanase is an important enzyme involved in the degradation of mannan, production of bioactive oligosaccharides, and biobleaching of kraft pulp. Mannanase must be thermostable for use in industrial applications. In a previous study, we found that the thermal stability of
María del Carmen Rodríguez-Gacio et al.
Journal of experimental botany, 63(11), 3976-3988 (2012-05-04)
The softening and degradation of the cell wall (CW), often mannan enriched, is involved in several processes during development of higher plants, such as meristematic growth, fruit ripening, programmed cell death, and endosperm rupture upon germination. Mannans are also the
Articles
Glycan Sequencing Using Exoglycosidases
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